Immune Assessment of BNT162b2 m-RNA-Spike Based Vaccine Response in Adults
| Autor: | Juan Irure-Ventura, Adriel Roa-Bautista, Mónica Renuncio-García, Elena González-López, Marcos López-Hoyos, Javier Gonzalo Ocejo-Vinyals, María Gutiérrez-Larrañaga, Marta Alonso-Peña, Patricia Lamadrid-Perojo, David Merino-Fernández, Sandra Guiral-Foz, David San Segundo, Alejandra Comins-Boo |
|---|---|
| Přispěvatelé: | Universidad de Cantabria |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
QH301-705.5 Medicine (miscellaneous) Stimulation General Biochemistry Genetics and Molecular Biology Article Flow cytometry 03 medical and health sciences 0302 clinical medicine Immune system Medicine CD134 IL-2 receptor Biology (General) B-specific response Vaccines biology medicine.diagnostic_test business.industry SARS-CoV-2 Vaccine efficacy T-specific response Vaccination 030104 developmental biology 030220 oncology & carcinogenesis Immunology biology.protein T follicular helper cells Antibody business |
| Zdroj: | Biomedicines . 2021 Jul 22;9(8):868 UCrea Repositorio Abierto de la Universidad de Cantabria Universidad de Cantabria (UC) Biomedicines, Vol 9, Iss 868, p 868 (2021) Biomedicines Volume 9 Issue 8 |
| Popis: | Vaccine efficacy is based on clinical data. Currently, the assessment of immune response after SARS-CoV-2 vaccination is scarce. A total of 52 healthcare workers were immunized with the same lot of BNT162b2 vaccine. The immunological response against the vaccine was tested using a T-specific assay based on the expression of CD25 and CD134 after stimulation with anti-N, -S, and -M specific peptides of SARS-CoV-2. Moreover, IgG anti-S2 and -RBD antibodies were detected using ELISA. Furthermore, the cell subsets involved in the response to the vaccine were measured in peripheral blood by flow cytometry. Humoral-specific responses against the vaccine were detected in 94% and 100% after the first and second doses, respectively. Therefore, anti-S T-specific responses were observed in 57% and 90% of the subjects after the first and second doses of the vaccine, respectively. Thirty days after the second dose, significant increases in T helper 1 memory cells (p < 0.001), peripheral memory T follicular helper (pTFH) cells (p < 0.032), and switched memory (p = 0.005) were observed. This study describes the specific humoral and cellular immune responses after vaccination with the new mRNA-based BNT162b2 vaccine. A mobilization of TFH into the circulation occurs, reflecting a specific activation of the immune system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|