Comparative proteomic analysis ofMethanothermobacter thermautotrophicusreveals methane formation from H2andCO 2under different temperature conditions
| Autor: | Xiaojue Peng, Lihui Mao, Yaohui Cai, Cong Liu, Yuan Jiangan, Xia Ding, Hongwei Xie, Xiongmin Zheng, Beijuan Hu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030102 biochemistry & molecular biology biology Methanogenesis Thermophile methane formation lcsh:QR1-502 Methanothermobacter thermautotrophicus Atmospheric temperature range Proteomics biology.organism_classification Microbiology lcsh:Microbiology Methane 03 medical and health sciences chemistry.chemical_compound proteomics temperature stress 030104 developmental biology iTRAQ chemistry Biophysics Protein folding Biogenesis Archaea |
| Zdroj: | MicrobiologyOpen, Vol 8, Iss 5, Pp n/a-n/a (2019) |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.715 |
| Popis: | The growth of all methanogens is limited to a specific temperature range. However, Methanothermobacter thermautotrophicus can be found in a variety of natural and artificial environments, the temperatures of which sometimes even exceed the temperature growth ranges of thermophiles. As a result, the extent to which methane production and survival are affected by temperature remains unclear. To investigate the mechanisms of methanogenesis that Archaea have evolved to cope with drastic temperature shifts, the responses of Methanothermobacter thermautotrophicus to temperature were investigated under a high temperature growth (71°C) and cold shock (4°C) using Isobaric tags for relative and absolute quantitation (iTRAQ). The results showed that methane formation is decreased and that protein folding and degradation are increased in both high‐ and low‐temperature treatments. In addition, proteins predicted to be involved in processing environmental information processing and in cell membrane/wall/envelope biogenesis may play key roles in affecting methane formation and enhancing the response of M. thermautotrophicus to temperature stress. Analysis of the genomic locations of the genes corresponding to these temperature‐dependent proteins predicted that 77 of the genes likely to form 32 gene clusters. Here, we assess the response of M. thermautotrophicus to different temperatures and provide a new level of understanding of methane formation and cellular putative adaptive responses. |
| Databáze: | OpenAIRE |
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