Decoy Cells versus Plasma Real-Time Polymerase Chain Reaction for the Detection of Polyomaviruses in Renal Transplant Patients: A Single Institutional Experience
| Autor: | Thuraya M Al Maskari, Najat Aldairi, Nasar Alwahaibi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Histology real-time polymerase chain reaction Urology urine samples 030209 endocrinology & metabolism Urine Decoy cells Pathology and Forensic Medicine law.invention 03 medical and health sciences renal transplants 0302 clinical medicine law Patient age polyomaviruses medicine lcsh:QH573-671 Polymerase chain reaction lcsh:Cytology business.industry Retrospective cohort study Gold standard (test) Real-time polymerase chain reaction Renal transplant 030220 oncology & carcinogenesis Original Article medicine.symptom business |
| Zdroj: | Journal of Cytology Journal of Cytology, Vol 37, Iss 1, Pp 30-33 (2020) |
| ISSN: | 0974-5165 0970-9371 |
| Popis: | Background and aims Microscopic detection of decoy cells is routinely performed in urine samples from renal transplant patients for the evaluation of polyomaviruses. However, they are scanty papers evaluated the diagnostic accuracy of decoy cells in urine samples. The aim of this study is to evaluate the diagnostic accuracy of decoy cells in urine samples and compare with plasma real-time polymerase chain reaction (RT-PCR) as a gold standard method. In addition, to compare the findings of this study with other similar studies. Methods A retrospective study over a period of four years from January 2014 to December 2017 was performed. A total of 89 urine samples from renal transplant patients were assessed for the presence of polyomaviruses and compared with plasma RT-PCR. The sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) were measured. Results There were 29 males and 18 females. The mean patient age was 40.3 years. The sensitivity, specificity, accuracy, PPV and NPV were 86.6%, 67.5%, 70.7%, 35.1% and 96.1%, respectively. Other similar studies reported a sensitivity of 41.9-84.6%, specificity of 65.8-100% and accuracy of 69.9-82%. Conclusion The findings of this study show that the detection of decoy cells in urine samples is a sensitive screening method for polyomaviruses. The findings of this study are compatible with other similar studies. |
| Databáze: | OpenAIRE |
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