IgG and IgA subclass distribution of total immunoglobulin and rheumatoid factors in rheumatoid tissue plasma cells
| Autor: | Tom Eirik Mollnes, F. Vartdal, Ove J. Mellbye, J. Pahle |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
Immunology Plasma Cells Immunoglobulins Antigen-Antibody Complex Complement Membrane Attack Complex Immunofluorescence Monoclonal antibody Subclass Arthritis Rheumatoid Psoriatic arthritis Rheumatology Rheumatoid Factor medicine Immunology and Allergy Rheumatoid factor Humans medicine.diagnostic_test business.industry Synovial Membrane General Medicine medicine.disease Complement system Immunoglobulin A medicine.anatomical_structure Rheumatoid arthritis Immunoglobulin G Synovial membrane business |
| Zdroj: | Scandinavian journal of rheumatology. 19(5) |
| ISSN: | 0300-9742 |
| Popis: | The subclass distribution of IgG and IgA plasma cells, and in IgG and IgA rheumatoid factor (RF) producing cells was studied in sections of synovial tissue from seropositive RA and various types of seronegative arthritis, including ankylosing spondylitis, psoriatic arthritis, and Reiter's syndrome. The study was performed with immunofluorescence technique and monoclonal IgG and IgA subclass specific antibodies. IgG RF producing cells were identified by their ability to bind and activate factors both in the early (C3) and late (C5b-9) part of the complement cascade. IgA RF cells were identified by double staining experiments with heat-aggregated IgG and monoclonal antibodies to IgA subclasses. In 23 tissues tested for total IgG, IgG1 cells were usually predominant, while the frequency of IgG3 cells was usually higher than that of IgG2. In 19 tissues also tested for IgA, both IgA subclasses were present in all tissues. IgA1 plasma cells were always predominant, with a mean ratio of IgA1 to IgA2 cells of approximately 10. In the 13 tissues tested for RF-producing cells, the highest frequency of IgG RF cells was found among the IgG3 cells, followed by IgG1 and IgG2. IgA RF cells were found in only one case, all cells being IgA1. |
| Databáze: | OpenAIRE |
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