Effects of chloroquine on GFAP, PCNA and cyclin D1 in hippocampus and cerebral cortex of rats with seizures induced by pentylenetetrazole
| Autor: | Wang Wei, Zhang Shu-hua, Liu Qingying, Zhu Changgeng |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Biomedical Engineering Hippocampus Biochemistry Rats Sprague-Dawley Biomaterials Random Allocation Epilepsy Cyclin D1 Seizures Chloroquine Proliferating Cell Nuclear Antigen Internal medicine Glial Fibrillary Acidic Protein Genetics medicine Animals Earth-Surface Processes Cerebral Cortex Glial fibrillary acidic protein biology medicine.disease Rats Proliferating cell nuclear antigen medicine.anatomical_structure Anticonvulsant Endocrinology nervous system Cerebral cortex Immunology biology.protein Pentylenetetrazole medicine.drug |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 25:625-628 |
| ISSN: | 1993-1352 1672-0733 |
| DOI: | 10.1007/bf02896153 |
| Popis: | The effects of chloroquine on glial fibrillary acidic protein (GFAP), proliferation cell nuclear antigen (PCNA) and Cyclin D1 in hippocampus and cerebral cortex of rats with seizures induced by pentylenetetrazole (PTZ) were observed in the present study. Forty-eight male adult Sprague-Dawley (SD) rats were randomly divided into control group, chloroquine intervening group, and PTZ group. The behavior and electroencephalogram (EEG) were observed and recorded. GFAP and PCNA were examined with immunohistochemistry. The content of Cyclin D1 in hippocampus and cerebral cortex was inspected with Western blot. The results showed no seizure activity in the control group, severe seizure activity in the PTZ group (IV - V degree), and slight seizure activity (I - IIl degree) in the chloroquine intervening group (P0.05). EEG recordings showed no epileptic spikes in the control group, high amplitude with fast frequency in the PTZ group, low-amplitude and slow frequency in the chloroquine intervening group. The expression of GFAP and the positive index of PCNA in the PTZ group were higher than those of control group (P0.05 and P0.01, respectively). No differences in GFAP expression and PCNA index were observed between chloroquine intervening and control groups (P0.05). The content of Cyclin D1 in hippocampus and cerebral cortex was significantly higher in the PTZ group than in control and chloroquine intervening groups (P0.05). Therefore, it is considered that chloroquine, by inhibiting the functions and proliferation of glial cells in the hippocampus and cerebral cortex, can alleviate the seizure activities. These results suggest that chloroquine may be an ideal anticonvulsant in preventing and treating epilepsy. |
| Databáze: | OpenAIRE |
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