Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function
| Autor: | Dileep Varma, Denis L. Dujardin, Richard B. Vallee, Stephanie A. Stehman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cytoplasm
Microinjections Endosome Chromosomal Proteins Non-Histone Dynein Golgi Apparatus Cell Cycle Proteins Biology 03 medical and health sciences symbols.namesake Microtubule Dynein ATPase Report Chlorocebus aethiops Animals Humans Kinetochores Interphase Research Articles 030304 developmental biology Organelles 0303 health sciences Kinetochore 030302 biochemistry & molecular biology Dyneins Cell Biology Golgi apparatus Cell biology ZW10 COS Cells symbols Dynactin RNA Interference Microtubule-Associated Proteins HeLa Cells |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Zeste white 10 (ZW10) is a mitotic checkpoint protein and the anchor for cytoplasmic dynein at mitotic kinetochores, though it is expressed throughout the cell cycle. We find that ZW10 localizes to pericentriolar membranous structures during interphase and cosediments with Golgi membranes. Dominant-negative ZW10, anti-ZW10 antibody, and ZW10 RNA interference (RNAi) caused Golgi dispersal. ZW10 RNAi also dispersed endosomes and lysosomes. Live imaging of Golgi, endosomal, and lysosomal markers after reduced ZW10 expression showed a specific decrease in the frequency of minus end–directed movements. Golgi membrane–associated dynein was markedly decreased, suggesting a role for ZW10 in dynein cargo binding during interphase. We also find ZW10 enriched at the leading edge of migrating fibroblasts, suggesting that ZW10 serves as a general regulator of dynein function throughout the cell cycle. |
| Databáze: | OpenAIRE |
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