TRPM7-Mediated Calcium Transport in HAT-7 Ameloblasts

Autor: Róbert Rácz, Anna Földes, Ákos Zsembery, Pamela DenBesten, Viktória Juhász, Erzsébet Kató, Kristóf Kádár, Gábor Varga, Yan Zhang, Heike Löchli, Martin C. Steward, László Köles
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
magnesium
lcsh:Chemistry
Mice
0302 clinical medicine
Ameloblasts
Anilides
lcsh:QH301-705.5
Spectroscopy
Mibefradil
Chemistry
pH
enamel
General Medicine
Amelogenesis
Hydrogen-Ion Concentration
Store-operated calcium entry
Naltrexone
Computer Science Applications
Cell biology
Incisor
TRPM7 channel protein
Ameloblast
Ion Channel Gating
Intracellular
medicine.drug
amelogenesis
TRPM Cation Channels
calcium entry
Models
Biological
Catalysis
Article
Cell Line
Inorganic Chemistry
03 medical and health sciences
Thiadiazoles
medicine
Extracellular
Animals
Humans
Physical and Theoretical Chemistry
Molecular Biology
Ion Transport
Organic Chemistry
store-operated calcium entry
Rats
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Naltriben
Calcium
030217 neurology & neurosurgery
Homeostasis
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 8
International Journal of Molecular Sciences, Vol 22, Iss 3992, p 3992 (2021)
ISSN: 1422-0067
Popis: TRPM7 plays an important role in cellular Ca2+, Zn2+ and Mg2+ homeostasis. TRPM7 channels are abundantly expressed in ameloblasts and, in the absence of TRPM7, dental enamel is hypomineralized. The potential role of TRPM7 channels in Ca2+ transport during amelogenesis was investigated in the HAT-7 rat ameloblast cell line. The cells showed strong TRPM7 mRNA and protein expression. Characteristic TRPM7 transmembrane currents were observed, which increased in the absence of intracellular Mg2+ ([Mg2+]i), were reduced by elevated [Mg2+]i, and were inhibited by the TRPM7 inhibitors NS8593 and FTY720. Mibefradil evoked similar currents, which were suppressed by elevated [Mg2+]i, reducing extracellular pH stimulated transmembrane currents, which were inhibited by FTY720. Naltriben and mibefradil both evoked Ca2+ influx, which was further enhanced by the acidic intracellular conditions. The SOCE inhibitor BTP2 blocked Ca2+ entry induced by naltriben but not by mibefradil. Thus, in HAT-7 cells, TRPM7 may serves both as a potential modulator of Orai-dependent Ca2+ uptake and as an independent Ca2+ entry pathway sensitive to pH. Therefore, TRPM7 may contribute directly to transepithelial Ca2+ transport in amelogenesis.
Databáze: OpenAIRE
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