Monitoring cellulose oxidation for protein immobilization in paper-based low-cost biosensors
Autor: | Leticia Jordão Marques de Oliveira, Emanuel Carrilho, Thiago Pinotti Segato, Amanda Imamura, Ayaz Hassan, Frank Nelson Crespilho |
---|---|
Rok vydání: | 2019 |
Předmět: |
Paper
Serum Albumin Human 02 engineering and technology Biosensing Techniques 01 natural sciences Proof of Concept Study Analytical Chemistry chemistry.chemical_compound Microscopy medicine Animals Humans Fourier transform infrared spectroscopy Cellulose Coloring Agents Detection limit Chromatography 010401 analytical chemistry Periodic Acid Periodate Serum Albumin Bovine 021001 nanoscience & nanotechnology Human serum albumin 0104 chemical sciences Immobilized Proteins chemistry Covalent bond Cattle Colorimetry 0210 nano-technology Biosensor Oxidation-Reduction medicine.drug |
Zdroj: | Mikrochimica acta. 187(5) |
ISSN: | 1436-5073 |
Popis: | The oxidation of paper by periodate was investigated and systematically characterized by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy, X-ray diffraction, goniometry, and dynamic mechanical analysis. For the first time, in situ FTIR microscopy analysis was performed, yielding chemical images of carbonyl groups on the cellulose fibers. The enhancement of protein immobilization on oxidized paper was quantified by a colorimetric assay with Ponceau dye, demonstrating that 0.5-h oxidation suffices to functionalize the paper-based devices. The oxidized paper was applied as a sensor for protein quantification in urine, a test able to detect levels of proteinuria and even microalbuminuria. The quantification was based on the capture of proteins through covalent bonds formed with the carbonyl groups on the oxidized paper followed by the staining of the region with Ponceau dye. There is a linear dependency between human serum albumin (HSA) concentration and the length of the stained blot from 0.1 to 3 mg mL−1. This method correlated linearly with a reference method showing a higher sensitivity (0.866 cm mL mg−1) than the latter. The limit of quantification was 0.1 mg mL−1, three times lower than that of the commercial strip. |
Databáze: | OpenAIRE |
Externí odkaz: |