P-glycoprotein (PGP) and lung resistance-related protein (LRP) expression and function in leukaemic blast cells
| Autor: | Francesco Lauria, Mariagrazia Michieli, Renato Fanin, Domenico Russo, Anna Ermacora, Michele Baccarani, Daniela Damiani, Paola Masolini, Donatella Raspadori, Angela Michelutti, Stefania Grimaz |
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| Rok vydání: | 1997 |
| Předmět: |
Myeloid
endocrine system diseases Daunorubicin Biology Rhodamine 123 chemistry.chemical_compound hemic and lymphatic diseases Acute lymphocytic leukemia Precursor cell polycyclic compounds medicine Humans Cytotoxic T cell ATP Binding Cassette Transporter Subfamily B Member 1 Lymphocytes Vault Ribonucleoprotein Particles P-glycoprotein Leukemia integumentary system Rhodamines Hematology medicine.disease Molecular biology Drug Resistance Multiple Neoplasm Proteins Multiple drug resistance medicine.anatomical_structure chemistry Drug Resistance Neoplasm Immunology biology.protein ATP-Binding Cassette Transporters lipids (amino acids peptides and proteins) Multidrug Resistance-Associated Proteins medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1365-2141 0007-1048 |
| Popis: | P-glycoprotein (PGP) lung resistance protein (LRP) and multidrug resistance associated protein (MRP) expressions and function were evaluated by now cytometry in 65 leukaemic patients (38 acute non-lymphocytic leukaemias, eight acute lymphocytic leukaemias, 19 Ph-positive chronic myeloid leukaemias in blastic phase). By using the MRK-16, the LRP-56 and the MRPm6 MoAbs, 34% of the cases did not over-express any proteins (-); 24.5% over-expressed (+) only PGP. 11% only LRP, 1.5% only MRP, 24.5%, both PGP and LRP, and 4.5% both PGP and MRP. The mean intracellular daunorubicin accumulation (IDA) and rhodamine 123 (Rh123) retention in the presence or absence of the reversal agent SDZ PSC 833 (PSC) of the PGP - /LRP - /MRP - cases were comparable to the ones observed in normal leucocytes. With respect to the non-over-expressing cases, the PGP - /LRP + /MRP - cases showed only an impaired IDA (mean 204 ± 29; P < 0.001). The PGP + /LRP + /MRP - cases had a defect both in IDA (mean 166±47, P |
| Databáze: | OpenAIRE |
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