Patronin-mediated minus end growth is required for dendritic microtubule polarity
| Autor: | Matthew Shorey, Richard M. Albertson, Chengye Feng, Alexis T. Weiner, Melissa M. Rolls, Kavitha Rao, Alvaro Sagasti, Pankajam Thyagarajan, Dylan Y. Seebold, Daniel J. Goetschius |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Embryo
Nonmammalian Embryonic Development Kinesins Dendrite Medical and Health Sciences Microtubules Article 03 medical and health sciences 0302 clinical medicine Microtubule Commentaries medicine Animals Drosophila Proteins Spotlight Zebrafish Research Articles 030304 developmental biology Microtubule nucleation Neurons 0303 health sciences Polarity (international relations) Nonmammalian biology Dendrite branch Cell Polarity Cell Biology Kinesin Dendrites Biological Sciences biology.organism_classification Dendritic microtubule Microtubule minus-end medicine.anatomical_structure Drosophila melanogaster Embryo Biophysics Microtubule-Associated Proteins 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | The Journal of Cell Biology The Journal of cell biology, vol 218, iss 7 |
| ISSN: | 1540-8140 |
| Popis: | Feng et al. describe persistent neuronal microtubule minus end growth that depends on the CAMSAP protein Patronin and is needed for dendritic minus-end-out polarity. Microtubule minus ends are thought to be stable in cells. Surprisingly, in Drosophila and zebrafish neurons, we observed persistent minus end growth, with runs lasting over 10 min. In Drosophila, extended minus end growth depended on Patronin, and Patronin reduction disrupted dendritic minus-end-out polarity. In fly dendrites, microtubule nucleation sites localize at dendrite branch points. Therefore, we hypothesized minus end growth might be particularly important beyond branch points. Distal dendrites have mixed polarity, and reduction of Patronin lowered the number of minus-end-out microtubules. More strikingly, extra Patronin made terminal dendrites almost completely minus-end-out, indicating low Patronin normally limits minus-end-out microtubules. To determine whether minus end growth populated new dendrites with microtubules, we analyzed dendrite development and regeneration. Minus ends extended into growing dendrites in the presence of Patronin. In sum, our data suggest that Patronin facilitates sustained microtubule minus end growth, which is critical for populating dendrites with minus-end-out microtubules. Graphical Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|