UV-Pre-Treated and Protein-Adsorbed Titanium Implants Exhibit Enhanced Osteoconductivity

Autor: Makoto Hirota, Takashi Taniyama, Takahiro Ogawa, Takayuki Ikeda, Hiroaki Kitajima, Juri Saruta, Yoshihiko Sugita
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Bone Regeneration
Cell Culture Techniques
Bone Morphogenetic Protein 2
02 engineering and technology
osteoconductivity
lcsh:Chemistry
0302 clinical medicine
UV-photofunctionalization
Ultraviolet light
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
Titanium
biology
Chemistry
Osteoblast
General Medicine
respiratory system
021001 nanoscience & nanotechnology
Computer Science Applications
medicine.anatomical_structure
osteoblast
Alkaline phosphatase
titanium implants
0210 nano-technology
Surface Properties
Ultraviolet Rays
chemistry.chemical_element
Mesenchymal Stem Cell Transplantation
Bone morphogenetic protein 2
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Adsorption
fibronectin
mechanical anchorage
medicine
Cell Adhesion
Animals
Physical and Theoretical Chemistry
Molecular Biology
Osteoblasts
Organic Chemistry
technology
industry
and agriculture
Mesenchymal Stem Cells
030206 dentistry
Alkaline Phosphatase
equipment and supplies
Fibronectins
Rats
Fibronectin
lcsh:Biology (General)
lcsh:QD1-999
Bone Substitutes
biology.protein
bone morphogenetic protein-2
Nuclear chemistry
Protein adsorption
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 12
International Journal of Molecular Sciences, Vol 21, Iss 4194, p 4194 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21124194
Popis: Titanium materials are essential treatment modalities in the medical field and serve as a tissue engineering scaffold and coating material for medical devices. Thus, there is a significant demand to improve the bioactivity of titanium for therapeutic and experimental purposes. We showed that ultraviolet light (UV)-pre-treatment changed the protein-adsorption ability and subsequent osteoconductivity of titanium. Fibronectin (FN) adsorption on UV-treated titanium was 20% and 30% greater after 1-min and 1-h incubation, respectively, than that of control titanium. After 3-h incubation, FN adsorption on UV-treated titanium remained 30% higher than that on the control. Osteoblasts were cultured on titanium disks after 1-h FN adsorption with or without UV-pre-treatment and on titanium disks without FN adsorption. The number of attached osteoblasts during the early stage of culture was 80% greater on UV-treated and FN-adsorbed (UV/FN) titanium than on FN-adsorbed (FN) titanium
osteoblasts attachment on UV/FN titanium was 2.6- and 2.1-fold greater than that on control- and UV-treated titanium, respectively. The alkaline phosphatase activity of osteoblasts on UV/FN titanium was increased 1.8-, 1.8-, and 2.4-fold compared with that on FN-adsorbed, UV-treated, and control titanium, respectively. The UV/FN implants exhibited 25% and 150% greater in vivo biomechanical strength of bone integration than the FN- and control implants, respectively. Bone morphogenetic protein-2 (BMP-2) adsorption on UV-treated titanium was 4.5-fold greater than that on control titanium after 1-min incubation, resulting in a 4-fold increase in osteoblast attachment. Thus, UV-pre-treatment of titanium accelerated its protein adsorptivity and osteoconductivity, providing a novel strategy for enhancing its bioactivity.
Databáze: OpenAIRE
Externí odkaz: