Comparative Study of Immunogenic Properties of Purified Capsular Polysaccharides from Streptococcus suis Serotypes 3, 7, 8, and 9: the Serotype 3 Polysaccharide Induces an Opsonizing IgG Response
| Autor: | Mariela Segura, Marcelo Gottschalk, Evgeny Vinogradov, Dominic Dolbec, Jean-Philippe Auger, Guillaume Goyette-Desjardins, Daisuke Takamatsu, Masatoshi Okura, Marie-Rose Van Calsteren |
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| Přispěvatelé: | Palmer, Guy H. |
| Rok vydání: | 2020 |
| Předmět: |
Serotype
0303 health sciences Streptococcus suis 030306 microbiology Secondary infection Immunogenicity Immunology serotype 3 immunogenicity Biology biology.organism_classification Microbiology capsular polysaccharide 3. Good health 03 medical and health sciences TLR2 Infectious Diseases Immune system biology.protein Parasitology Titermax Antibody 030304 developmental biology |
| Zdroj: | Infection and Immunity. 88 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00377-20 |
| Popis: | Streptococcus suis is an encapsulated bacterium and one of the most important swine pathogens and a zoonotic agent for which no effective vaccine exists. Bacterial capsular polysaccharides (CPSs) are poorly immunogenic, but anti-CPS antibodies are essential to the host defense against encapsulated bacteria. In addition to the previously known serotypes 2 and 14, which are nonimmunogenic, we have recently purified and described the CPS structures for serotypes 1, 1/2, 3, 7, 8, and 9. Here, we aimed to elucidate how these new structurally diverse CPSs interact with the immune system to generate anti-CPS antibody responses. CPS-stimulated dendritic cells produced significant levels of C–C motif chemokine ligand 3 (CCL3), partially via Toll-like receptor 2 (TLR2)- and myeloid differentiation factor 88-dependent pathways, and CCL2, via TLR-independent mechanisms. Mice immunized with purified serotype 3 CPS adjuvanted with TiterMax Gold produced an opsonizing IgG response, whereas other CPSs or adjuvants were negative. Mice hyperimmunized with heat-killed S. suis serotypes 3 and 9 both produced anti-CPS type 1 IgGs, whereas serotypes 7 and 8 remained negative. Also, mice infected with sublethal doses of S. suis serotype 3 produced primary anti-CPS IgM and IgG responses, of which only IgM were boosted after a secondary infection. In contrast, mice sublethally infected with S. suis serotype 9 produced weak anti-CPS IgM and IgG responses following a secondary infection. This study provides important information on the divergent evolution of CPS serotypes with highly different structural and/or biochemical properties within S. suis and their interaction with the immune system. |
| Databáze: | OpenAIRE |
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