Assessment of bacteriophage vB_Pd_PDCC-1 on bacterial dynamics during ontogenetic development of the longfin yellowtail (Seriola rivoliana)
| Autor: | Bernardo Veyrand-Quirós, Laura T. Guzmán-Villanueva, Ana G. Reyes, Dariel Tovar-Ramírez, Eduardo Quiroz-Guzmán, Joan Salas-Leiva, José Luis Balcázar, Carmen Rodríguez-Jaramillo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Phage therapy
medicine.medical_treatment Zoology Applied Microbiology and Biotechnology Bacteriophage 03 medical and health sciences RNA Ribosomal 16S Gammaproteobacteria medicine Animals Bacteriophages 030304 developmental biology Vibrio 0303 health sciences biology 030306 microbiology Seriola rivoliana Fishes Seriola General Medicine biology.organism_classification Flavobacteriaceae Longfin Myoviridae Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 105(7) |
| ISSN: | 1432-0614 |
| Popis: | The Seriola genus includes species of worldwide commercial importance due to its rapid growth and easy adaptability to confinement conditions. However, like other fish species, large mortalities occur during their early life stages, where the main problems are caused by opportunistic bacteria. Disease control strategies are thus urgently needed. The present study aimed to evaluate the efficacy of phage vB_Pd_PDCC-1 during the early development of longfin yellowtail (Seriola rivoliana), as well as its effect on microbial communities. This broad-host-range phage was added to the culture every 3 days starting from the egg-stage until 12 days after hatching (DAH) at a concentration of 1.41×1010 plaque-forming units (PFU) per mL and at a multiplicity of infection (MOI) of 1. The results showed positive effects (p 0.05) in the composition and structure of the associated microbiota. However, sequences affiliated to the Gammaproteobacteria class were displaced by those belonging to the Alphaproteobacteria class over time regardless of the treatment received. At the family level, there was a decrease in Rhodobacteraceae, Pseudoalteromonadaceae, and Flavobacteriaceae in both groups over time. To our best knowledge, this study represents the first attempt to evaluate the effect of a phage as a biological control agent during ontogenetic development of longfin yellowtail larvae. • Phages can be used against proliferation of Vibrio in fish cultures. • Seriola includes several important commercial fish species due to its rapid growth. • Phages do not cause significant changes in the associated microbiota. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink