Influence of lyophilization, fluidized bed drying, addition of protectants, and storage on the viability of lactic acid bacteria
| Autor: | M. Geppl, H. Danner, Rudolf Braun, Markus Neureiter, S. Strasser |
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| Rok vydání: | 2009 |
| Předmět: |
Sucrose
Cell Survival Enterococcus faecium Cell Culture Techniques Colony Count Microbial Applied Microbiology and Biotechnology chemistry.chemical_compound Cryoprotective Agents Starter Polysaccharides Food science Desiccation Cryopreservation Dehydration biology Temperature Trehalose food and beverages General Medicine biology.organism_classification Maltodextrin Culture Media Lactic acid Freeze Drying Glucose chemistry Biochemistry Lactobacillus plantarum Bacteria Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 107:167-177 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | Aims: The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. Methods and Results: After incubation with the protectants glucose, sucrose, trehalose, and maltodextrin the concentrated bacterial suspensions were subjected to fluidized bed drying and lyophilization and subsequently stored at 4, 22, and 35°C for half a year. Lactobacillus plantarum turned out to be more sensitive to both drying methods than Ent. faecium. Without the addition of a protectant cells of both strains suffered higher losses during fluidized bed drying. Elevated storage temperatures correlate with a higher decline of viable bacterial cells. Conclusions: Although survival rates varied between the strains, the nonreducing disaccharides revealed overall best protection for both investigated lactic acid bacteria during processing and storage. The addition of protective carbohydrates can prevent the decline in viability during fluidized bed drying. Significance and Impact of the Study: The influence of protectants proved to be species specific and therefore needs to be determined on a case-to-case basis. Survival rates, duration, and energy consumption appear to be the crucial parameters to evaluate the economy of production processes for industrial starter cultures. |
| Databáze: | OpenAIRE |
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