Loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in human endometrial cancer
| Autor: | Takumi Konno, Syunta Takahashi, Seiro Satohisa, Tsuyoshi Saito, Tsubasa Hatakeyama, Yakuto Kaneko, Chihiro Arimoto, Takuya Kakuki, Hiroshi Shimada, Shyuetsu Abe, Takashi Kojima, Takayuki Kohno, Kenichi Takano |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell Cycle Proteins Amphiregulin Article Tight Junctions 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor Humans RNA Small Interfering Cell Proliferation Receptors Lipoprotein Multidisciplinary Chemistry Cell growth Microfilament Proteins Tricellular tight junction Membrane Proteins Nuclear Proteins TEA Domain Transcription Factors Cell migration Epithelial Cells Angiomotin Endometrial Neoplasms Merlin (protein) DNA-Binding Proteins Gene Expression Regulation Neoplastic 030104 developmental biology Angiomotins 030220 oncology & carcinogenesis Cancer cell Cancer research Intercellular Signaling Peptides and Proteins Female Transcription Factors |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Lipolysis-stimulated lipoprotein receptor (LSR) is a unique molecule of tricellular contacts of normal and cancer cells. We investigated how the loss of LSR induced cell migration, invasion and proliferation in endometrial cancer cell line Sawano. mRNAs of amphiregulin (AREG) and TEA domain family member 1 (TEAD1) were markedly upregulated by siRNA-LSR. In endometrial cancer tissues, downregulation of LSR and upregulation of AREG were observed together with malignancy, and Yes-associated protein (YAP) was present in the nuclei. siRNA-AREG prevented the cell migration and invasion induced by siRNA-LSR, whereas treatment with AREG induced cell migration and invasion. LSR was colocalized with TRIC, angiomotin (AMOT), Merlin and phosphorylated YAP (pYAP). siRNA-LSR increased expression of pYAP and decreased that of AMOT and Merlin. siRNA-YAP prevented expression of the mRNAs of AREG and TEAD1, and the cell migration and invasion induced by siRNA-LSR. Treatment with dobutamine and 2-deoxy-D-glucose and glucose starvation induced the pYAP expression and prevented the cell migration and invasion induced by siRNA-LSR. siRNA-AMOT decreased the Merlin expression and prevented the cell migration and invasion induced by siRNA-LSR. The loss of LSR promoted cell invasion and migration via upregulation of TEAD1/AREG dependent on YAP/pYAP and AMOT/Merlin in human endometrial cancer cells. |
| Databáze: | OpenAIRE |
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