Comparative study of free respiration in liver mitochondria during oxidation of various electron donors and under conditions of shutdown of complex III of the respiratory chain

Autor:	Victor N. Samartsev, Alena A. Semenova, Andrey N. Ivanov, Mikhail V. Dubinin
Rok vydání:	2022
Předmět:	Respiration Succinic Acid Biophysics Glutamic Acid Electrons Mitochondria Liver Succinates Cell Biology Biochemistry Electron Transport Electron Transport Complex III Oxygen Consumption Protons Molecular Biology
Zdroj:	Biochemical and Biophysical Research Communications. 606:163-167
ISSN:	0006-291X
Popis:	The present work shows that the rate of free respiration of liver mitochondria (in the absence of ATP synthesis (state 4) during the oxidation of succinate is 1.7 times higher than during the oxidation of glutamate with malate. In turn, in the case of oxidation of ferrocyanide with ascorbate, this value is 3.1 times greater than in the case of succinate oxidation. A similar pattern is also observed upon stimulation of free respiration by low concentrations (5 and 10 μM) of the protonophore uncoupler 2,4-dinitrophenol (DNP). It is found that the passive leakage rate of protons in state 4 is the same if the H
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b37903642671ac3b53c21497f96ef144 https://doi.org/10.1016/j.bbrc.2022.03.099 Zobrazit plný text záznamu Full Text from ScienceDirect