Vertical Flow Cellulose-Based Assays for SARS-CoV-2 Antibody Detection in Human Serum
| Autor: | Emma H. Yee, Eric A. Miller, Yining Hao, Peter R. Preiser, Dousabel M Y Tay, Patthara Kongsuphol, Huan Jia, Seunghyeon Kim, Megan E. McBee, Hadley D. Sikes |
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| Rok vydání: | 2021 |
| Předmět: |
paper-based assay
serology Bioengineering 02 engineering and technology Antibodies Viral Sensitivity and Specificity 01 natural sciences Article Serology Antigen Seroepidemiologic Studies antibody vertical flow medicine Humans Seroprevalence Cellulose Pandemics Instrumentation cellulose-binding domain Point of care Immunoassay Fluid Flow and Transfer Processes medicine.diagnostic_test biology SARS-CoV-2 horseradish peroxidase Chemistry Process Chemistry and Technology 010401 analytical chemistry COVID-19 021001 nanoscience & nanotechnology Virology 0104 chemical sciences Nucleic acid biology.protein Antibody 0210 nano-technology |
| Zdroj: | ACS Sensors |
| ISSN: | 2379-3694 |
| DOI: | 10.1021/acssensors.1c00235 |
| Popis: | Rapid and inexpensive serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antibodies are essential to conduct large-scale seroprevalence surveys and can potentially complement nucleic acid or antigen tests at the point of care. During the COVID-19 pandemic, extreme demand for traditional lateral flow tests has stressed manufacturing capacity and supply chains. Motivated by this limitation, we developed a SARS-CoV-2 antibody test using cellulose, an alternative membrane material, and a double-antigen sandwich format. Functionalized SARS-CoV-2 antigens were used as both capture and reporter binders, replacing the anti-human antibodies currently used in lateral flow tests. The test could provide enhanced sensitivity because it labels only antibodies against SARS-CoV-2 and the signal intensity is not diminished due to other human antibodies in serum. Three-dimensional channels in the assay were designed to have consistent flow rates and be easily manufactured by folding wax-printed paper. We demonstrated that this simple, vertical flow, cellulose-based assay could detect SARS-CoV-2 antibodies in clinical samples within 15 min, and the results were consistent with those from a laboratory, bead-based chemiluminescence immunoassay that was granted emergency use approval by the US FDA. |
| Databáze: | OpenAIRE |
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