Corticosterone inhibits the expression of cannabinoid receptor‑1 and cannabinoid receptor agonist‑induced decrease in cell viability in glioblastoma cells
| Autor: | Takako Ohno‑Shosaku, Yoshibumi Ueda, Hiroyuki Nakamura, Naotoshi Sugimoto, Hiroaki Ishibashi, Akihiro Yachie |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Agonist Cancer Research medicine.medical_specialty Cannabinoid receptor medicine.drug_class Inflammation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Corticosterone Internal medicine medicine Viability assay Chemistry musculoskeletal neural and ocular physiology Articles Endocannabinoid system 030104 developmental biology Endocrinology nervous system Oncology 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) medicine.symptom Signal transduction psychological phenomena and processes |
| Zdroj: | Oncology Letters. |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | The endocannabinoid system regulates physiological and pathological conditions, including inflammation and cancer. Recently, emotional and physical stressors were observed to be involved in impairing the endocannabinoid system, which was concomitant with an increase in serum corticosteroids. However, the influence of corticosteroids on the endocannabinoid system has yet to be completely elucidated. The present study investigated the effects of corticosterone, one of the corticosteroids, on the endocannabinoid system in malignant glioblastoma cells in vitro. U-87 MG cells derived from malignant glioblastoma were subjected to corticosterone stimulation and their viability, signal transduction, and endocannabinoid-related gene expression were examined. Corticosterone decreased the mRNA and protein expressions of cyclooxygenase-2. Of note, although endocannabinoids decreased cell viability, corticosterone inhibited the cannabinoid receptor agonist-induced decrease in cell viability by downregulating the mRNA and protein expressions of cannabinoid receptor 1 (CB1) in glioblastoma cells. These results suggest that corticosteroids modify the endocannabinoid system in glioblastoma cells, and a reduction in the beneficial anti-tumor effects of endocannabinoids through downregulation of the CB1 receptor by corticosterone may promote the malignant phenotype of glioblastoma. |
| Databáze: | OpenAIRE |
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