Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery
| Autor: | Thomas Linemann, Torben Moos, Jens Christian Laursen, Kristian Gaarn du Jardin, Jacek Lichota, Louiza Bohn Thomsen, Jesper Bornø Jensen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Liposome endothelium Chemistry nanoparticle lcsh:RS1-441 Pharmaceutical Science magnetic field Transfection blood-brain barrier In vitro Article lcsh:Pharmacy and materia medica transfection In vivo PEG ratio Drug delivery medicine Biophysics Magnetofection Magnetic nanoparticles magnetofection |
| Zdroj: | Pharmaceutics Linemann, T, Thomsen, L B, du Jardin, K G, Laursen, J C, Jensen, J B, Lichota, J & Moos, T 2013, ' Development of a Novel Lipophilic, Magnetic Nanoparticle for in Vivo Drug Delivery ', Pharmaceutics, vol. 5, no. 2, pp. 246-260 . https://doi.org/10.3390/pharmaceutics5020246 Volume 5 Issue 2 Pages 246-260 Pharmaceutics, Vol 5, Iss 2, Pp 246-260 (2013) |
| ISSN: | 1999-4923 |
| Popis: | The aim of the present study was to evaluate the transfection potential of chitosan-coated, green-fluorescent magnetic nanoparticles (MNPs) (chi-MNPs) after encapsulation inside polyethylglycol (PEG)ylated liposomes that produced lipid-encapsulated chitosan-coated MNPs (lip-MNPs), and also to evaluate how these particles would distribute in vivo after systemic injection. The transfection potential of both chi-MNPs and lip-MNPs was evaluated in vitro in rat brain endothelial 4 (RBE4) cells with and without applying a magnetic field. Subsequently, the MNPs were evaluated in vivo in young rats. The in vitro investigations revealed that the application of a magnetic field resulted in an increased cellular uptake of the particles. The lip-MNPs were able to transfect the RBE4 cells with an incidence of approximately 20% of a commercial transfection agent. The in vivo distribution studies revealed that lip-MNPs had superior pharmacokinetic properties due to evasion of the RES, including hepatic Kuppfer cells and macrophages in the spleen. In conclusion, we were able to design a novel lipid-encapsulated MNP with the ability to carry genetic material, with favorable pharmacokinetic properties, and under the influence of a magnetic field with the capability to mediate transfection in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|