Prototype multi-biomarker test for point-of-care leprosy diagnostics
| Autor: | Abu Sufian Chowdhury, Khorshed Alam, Anouk van Hooij, Annemieke Geluk, Paul L. A. M. Corstjens, Elisa M. Tjon Kon Fat, Jong-Pill Kim, Marufa Khatun, Jan Hendrik Richardus, Danielle de Jong, Johan Chandra Roy, Santosh Soren |
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| Přispěvatelé: | Public Health |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Fingerstick Applied Microbiology Paucibacillary Leprosy 02 engineering and technology Article Lateral flow test 03 medical and health sciences SDG 3 - Good Health and Well-being medicine lcsh:Science Mycobacterium leprae Host protein Point of care Multidisciplinary Diagnostic Technique in Health Technology biology business.industry 021001 nanoscience & nanotechnology medicine.disease biology.organism_classification 030104 developmental biology Immunology Biomarker (medicine) lcsh:Q Leprosy 0210 nano-technology business Biotechnology |
| Zdroj: | iScience, 24(1). CELL PRESS iScience, 24(1):102006. Elsevier Inc. iScience iScience, Vol 24, Iss 1, Pp 102006-(2021) |
| ISSN: | 2589-0042 |
| Popis: | Summary To end the decade-long, obstinately stagnant number of new leprosy cases, there is an urgent need for field-applicable diagnostic tools that detect infection with Mycobacterium leprae, leprosy's etiologic agent. Since immunity against M. leprae is characterized by humoral and cellular markers, we developed a lateral flow test measuring multiple host proteins based on six previously identified biomarkers for various leprosy phenotypes. This multi-biomarker test (MBT) demonstrated feasibility of quantitative detection of six host serum proteins simultaneously, jointly allowing discrimination of patients with multibacillary and paucibacillary leprosy from control individuals in high and low leprosy endemic areas. Pilot testing of fingerstick blood showed similar MBT performance in point-of-care (POC) settings as observed for plasma and serum. Thus, this newly developed prototype MBT measures six biomarkers covering immunity against M. leprae across the leprosy spectrum. The MBT thereby provides the basis for immunodiagnostic POC tests for leprosy with potential for other (infectious) diseases as well. Graphical Abstract Highlights • Prototype MBT that quantitatively detects six host-derived biomarkers is developed • The immunopathological spectrum of leprosy is ideally suited to evaluate the MBT • MBT discriminated patients with leprosy from controls in a high and non-endemic area • Application of the MBT using low invasive fingerstick blood is technically feasible Diagnostic Technique in Health Technology; Applied Microbiology; Biotechnology |
| Databáze: | OpenAIRE |
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