Genome sequence of multidrug-resistant Erysipelothrix rhusiopathiae ZJ carrying several acquired antimicrobial resistance genes
| Autor: | Guang-xu Ma, Ju Gu, Ping Li, Xianjun Xie, Jinxin Liu, Anyun Zhang, Yunxia Li, Chang-Wen Xu, Chang-Wei Lei |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) China Swine Erysipelas Swine 030106 microbiology Immunology Erysipelothrix rhusiopathiae Multidrug resistance Genome Microbiology Integrative and conjugative element 03 medical and health sciences 0302 clinical medicine Plasmid Gene cluster Drug Resistance Bacterial Immunology and Allergy Animals 030212 general & internal medicine Gene Prophage Genetics Whole genome sequencing biology biology.organism_classification QR1-502 Anti-Bacterial Agents Multiple drug resistance Erysipelothrix |
| Zdroj: | Journal of Global Antimicrobial Resistance, Vol 21, Iss, Pp 13-15 (2020) |
| ISSN: | 2213-7173 |
| Popis: | Objectives This study aimed to determine the genetic environment of antimicrobial resistance genes (ARGs) in Erysipelothrix rhusiopathiae strain ZJ isolated from a pig with symptoms of swine erysipelas in China. Methods Illumina MiSeq (200× coverage) and PacBio RS II (100× coverage) platforms were used for genome sequencing. ARGs and prophages were identified using ResFinder 3.0 and PHASTER, respectively. A conjugation experiment, induced prophage infection and long-term passage assay were performed to determine the transferability and stability of ARGs in this strain. Results The assembled circular genome of E. rhusiopathiae ZJ was 1 945 689 bp with a GC content of 36.48%; no plasmid sequence was detected. Eleven acquired ARGs were identified in the genome. A novel integrative and conjugative element (ICE) encoding a multidrug resistance (MDR) gene cluster [aadE–apt–spw–lsa(E)–lnu(B)–aadE–sat4–aphA3] was identified in strain ZJ. A prophage Φ1605 harbouring mef(A)–msr(D) and tet(M) was also found in this strain, which can take a circular form and can be induced by mitomycin C to infect E. rhusiopathiae G4T10 for ARG transfer. Conclusion To our knowledge, this is the first report of a complete genome sequence of E. rhusiopathiae carrying multiple ARGs obtained from a pig farm. This is the first identification of a novel chimeric ICE carrying a MDR gene cluster and a prophage carrying ARGs in E. rhusiopathiae, which will provide a valuable reference to understand the potential transfer mechanism of MDR gene clusters carried by ICEs and prophages in Gram-positive bacteria. |
| Databáze: | OpenAIRE |
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