Helix packing in polytopic membrane proteins: the lactose permease of Escherichia coli
| Autor: | H. Ronald Kaback, Jianhua Wu, John C. Voss |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Lactose permease
Models Molecular Monosaccharide Transport Proteins Symporters Chemistry Escherichia coli Proteins Molecular Sequence Data Membrane Proteins Membrane Transport Proteins macromolecular substances Protein engineering medicine.disease_cause Protein Structure Secondary Protein Structure Tertiary Biochemistry Lac permease Membrane protein Structural Biology Helix medicine Escherichia coli Mutagenesis Site-Directed Amino Acid Sequence Molecular Biology |
| Zdroj: | Current opinion in structural biology. 7(4) |
| ISSN: | 0959-440X |
| Popis: | Recent advances in protein engineering have facilitated the development of alternative approaches to determine helix packing in polytopic membrane proteins. Using the lac permease as a paradigm, several site-directed biophysical and biochemical techniques are described which should be generally applicable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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