First Molecular Identification of Canine Parvovirus Type 2 (CPV2) in Chile Reveals High Occurrence of CPV2c Antigenic Variant
| Autor: | Castillo, C., Neira, V., Aniñir, P., Grecco Patiño, Sofía, Pérez Crossa, Ruben Gustavo, Panzera Crespo, Yanina, Zegpi, N. A., Sandoval, A., Sandoval, S., Cofre, S., Ortega, R., Bastos, A. |
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| Přispěvatelé: | Castillo C., Neira V., Aniñir P., Grecco Patiño Sofía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Pérez Crossa Ruben Gustavo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Panzera Crespo Yanina, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología., Zegpi N. A., Sandoval A., Sandoval S., Cofre S., Ortega R. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
genetic characterization lcsh:Veterinary medicine General Veterinary biology 040301 veterinary sciences Canine parvovirus 04 agricultural and veterinary sciences Canine parvovirus type 2 Brief Research Report South America biology.organism_classification Virology 0403 veterinary science 03 medical and health sciences CPV2c Antigen canine parvovirus type 2 lcsh:SF600-1100 Veterinary Science Chile Genetic characterization 030304 developmental biology Molecular identification |
| Zdroj: | Frontiers in Veterinary Science, Vol 7 (2020) Frontiers in Veterinary Science COLIBRI Universidad de la República instacron:Universidad de la República |
| ISSN: | 2297-1769 |
| DOI: | 10.3389/fvets.2020.00194/full |
| Popis: | Canine parvovirus type 2 (CPV2) is one of the most important intestinal pathogens in dogs and puppies. CPV2 has been evolved into three genetic and antigenic variants (2a, 2b, and 2c), which are distributed worldwide. We reported the first study of genetic diversity of CPV2 in Chile. Sixty-five samples were collected from puppies presenting with severe gastroenteritis and different vaccination statuses. PCR, restriction fragment length polymorphism (RFLP), and partial sequencing of the coding region of the structural viral protein VP2 was performed. Thirty of a total of 65 samples tested positive by PCR out of which 19 were further classified as CPV2c and one as CPV2a using RFLP and Sanger sequencing. The phylogeny was in concordance with the RFLP analysis. This is the first report of the genetic characterization of CPV2 in Chile and reveals a high occurrence of CPV2c. |
| Databáze: | OpenAIRE |
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