Biological impact of hepatitis B virus X-hepatitis C virus core fusion gene on human hepatocytes
| Autor: | Zhen Ma, Guo-Min Chen, Qin-Hai Shen, Da-Zhi Zhang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Hepatitis B virus
Carcinoma Hepatocellular Genes Viral viruses Hepacivirus Mice Nude Apoptosis medicine.disease_cause Adenoviridae Fusion gene Mice Cell Line Tumor medicine Animals Humans Viral Regulatory and Accessory Proteins Gene Mice Inbred BALB C Base Sequence biology Viral Core Proteins Cell Cycle Gastroenterology virus diseases General Medicine Cell cycle biology.organism_classification Virology Molecular biology digestive system diseases In vitro Basic Research DNA Viral Hepatocytes Trans-Activators Gene Fusion Hepatitis C Antigens |
| Zdroj: | World Journal of Gastroenterology. 14:5412 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.14.5412 |
| Popis: | AIM: To investigate the biological impact of hepatitis B virus X- hepatitis C virus core (HBV X-HCV C) fusion gene on hepatoma cells. METHODS: The recombinant adenoviruses Ad-XC, Ad-X and Ad-C expressing HBV X-HCV C fusion gene, HBV X gene and HCV C gene were constructed, respectively. Hepatoma cells were infected with different recombinant adenoviruses. MTT, colony-forming experiment, FCM, TUNEL assay were performed to observe the biological impact of the HBV X-HCV C fusion gene on liver cells. RESULTS: MTT showed that the Ad-XC group cells grew faster than the other group cells. Colony-forming experiment showed that the colony-forming rate for the Ad-XC group cells was significantly higher than that for the other group cells. FCM analysis showed that Ad-XC/Ad-X/Ad-C infection enhanced the progression of G1→S phase in the HepG2 cell cycle. The apoptosis index of the Ad-XC, Ad-X, Ad-C group cells was significantly lower than that of the Ad0 and control group cells. Semi-quantitative RT-PCR showed that the expression level of c-myc was the highest in Ad-XC infected cells. Tumor formation was found at the injected site of mice inoculated with Ad-XC-infected LO2 cells, but not in control mice. CONCLUSION: Ad-XC, Ad-X and Ad-C facilitate the proliferation activity of HepG2 cells and inhibit their apoptosis in vitro. The effect of Ad-XC is significantly stronger than that of Ad-X and Ad-C. Up-regulation of c-myc may be one of the mechanisms underlying the synergism of HBV X and HCV C genes on hepatocarcinogenesis in athymic nude mice. |
| Databáze: | OpenAIRE |
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