miR-378 inhibits cell growth and enhances apoptosis in human myelodysplastic syndromes
| Autor: | Jianxiang Chi, Tao Zhang, Xingyi Kuang, Zesong Yang, Li Wang, Chunmei Wei |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Cancer Research Myeloid Blotting Western Cell Apoptosis Mice SCID Biology Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Mice 03 medical and health sciences 0302 clinical medicine Mice Inbred NOD hemic and lymphatic diseases Biomarkers Tumor Tumor Cells Cultured medicine Animals Humans RNA Messenger Cell Proliferation Neoplasm Staging Reverse Transcriptase Polymerase Chain Reaction Cell growth Cell Cycle Myeloid leukemia Cell cycle Prognosis medicine.disease Xenograft Model Antitumor Assays Cell biology Leukemia Myeloid Acute MicroRNAs Leukemia 030104 developmental biology medicine.anatomical_structure Oncology Case-Control Studies Myelodysplastic Syndromes 030220 oncology & carcinogenesis Cancer research Female Bone marrow |
| Zdroj: | International Journal of Oncology. 49:1921-1930 |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2016.3689 |
| Popis: | miR-378 has been proven to inhibit cell growth, migration and invasion in different types of cancers. In this study, we found that miR-378 was commonly downregulated in the bone marrow cells obtained from myelodysplastic syndrome (MDS) patients. We further investigated the role of miR-378 in the proliferation and apoptosis of SKM-1 cells, an acute myeloid leukemia cell line established in the leukemic phase during the progression of MDS to AML (MDS/AML). Results indicated that overexpression of miR-378 in SKM-1 cells interfered with proliferation via inducing apoptosis and G0/G1-phase cell cycle arrest, and suppressive effect of miR-378 on MDS/AML cells may be mediated partly through Bcl-w and CDC40. Moreover, apoptosis induced by miR-378 correlated with increased expression of Bax and activation of caspase-3, -8 and -9. Taken together, our data support a critical role for miR-378 in the pathogenesis of MDS and provide a novel therapeutic target in this complex disease. |
| Databáze: | OpenAIRE |
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