Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors
| Autor: | Ahmad Manbohi, Seyed Ali Mousavi Shaegh, Thomas Shupe, Anthony Atala, Yu Shrike Zhang, Duckjin Kim, Colin E. Bishop, Mehmet R. Dokmeci, Antonia Silvestri, Huseyin Avci, Sukyoung Chae, Su Ryon Shin, Noor Shaikh, Aleksander Skardal, João Ribas, Parissa Alerasool, Giovanni Calzone, Alessandro Polini, Solange Massa, Weijia Zhang, Ali Khademhosseini, Tugba Kilic, Ning Hu, Erica Budina, Reza Riahi, Amir Sanati Nezhad, Jian Kang, Julio Aleman, Fabio De Ferrari, Adel Pourmand, Nupura S. Bhise |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Engineering Microfluidics Drug Evaluation Preclinical Nanotechnology 02 engineering and technology Biosensing Techniques Organ-on-a-chip Models Biological 03 medical and health sciences Automation Optical sensing Electrochemical biosensor Humans Microbioreactor Multidisciplinary business.industry Physical sensor Myocardium Heart Breadboard Modular design 021001 nanoscience & nanotechnology Organoids 030104 developmental biology Drug screening Liver PNAS Plus Embedded system Personalized medicine 0210 nano-technology business |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 114(12) |
| ISSN: | 1091-6490 |
| Popis: | Organ-on-a-chip systems are miniaturized microfluidic 3D human tissue and organ models designed to recapitulate the important biological and physiological parameters of their in vivo counterparts. They have recently emerged as a viable platform for personalized medicine and drug screening. These in vitro models, featuring biomimetic compositions, architectures, and functions, are expected to replace the conventional planar, static cell cultures and bridge the gap between the currently used preclinical animal models and the human body. Multiple organoid models may be further connected together through the microfluidics in a similar manner in which they are arranged in vivo, providing the capability to analyze multiorgan interactions. Although a wide variety of human organ-on-a-chip models have been created, there are limited efforts on the integration of multisensor systems. However, in situ continual measuring is critical in precise assessment of the microenvironment parameters and the dynamic responses of the organs to pharmaceutical compounds over extended periods of time. In addition, automated and noninvasive capability is strongly desired for long-term monitoring. Here, we report a fully integrated modular physical, biochemical, and optical sensing platform through a fluidics-routing breadboard, which operates organ-on-a-chip units in a continual, dynamic, and automated manner. We believe that this platform technology has paved a potential avenue to promote the performance of current organ-on-a-chip models in drug screening by integrating a multitude of real-time sensors to achieve automated in situ monitoring of biophysical and biochemical parameters. |
| Databáze: | OpenAIRE |
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