Macrophages in experimental rat lung isografts and allografts: infiltration and proliferation in situ
| Autor: | Andree Schmidt, Holger Garn, Gabriele Fuchs-Moll, Markus Hirschburger, Andreas Kaufmann, Veronika Grau, Winfried Padberg, Jochen Sucke, Petra Freitag |
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| Rok vydání: | 2006 |
| Předmět: |
Graft Rejection
Pathology medicine.medical_specialty medicine.medical_treatment Immunology In situ hybridization Biology Macrophages Alveolar medicine Immunology and Allergy Lung transplantation Animals Transplantation Homologous Lymphocytes Macrophage homeostasis Lung Macrophages Cell Biology Ectodysplasins medicine.disease Rats Transplantation Disease Models Animal Transplantation Isogeneic medicine.anatomical_structure Rats Inbred Lew Alveolar macrophage Infiltration (medical) CD163 Lung Transplantation |
| Zdroj: | Journal of leukocyte biology. 81(1) |
| ISSN: | 0741-5400 |
| Popis: | Alveolar macrophages (AMs) and peribronchial/perivascular macrophages are probably involved in lung allograft damage. We investigate leukocyte infiltration into graft tissue and address the question whether proliferation in situ contributes to macrophage homeostasis and accumulation. Lung transplantation was performed in the Lewis (LEW)-to-LEW and in the Dark Agouti-to-LEW rat strain combination. Graft infiltration by ED1+ and ED2+ (CD163) macrophages was analyzed by immunohistochemistry (IHC) and compared with infiltration by lymphocytes. Cells in the S-phase of the cell cycle were pulse-labeled with BrdU and detected immunohistochemically. Finally, the donor or recipient origin of AMs was determined by IHC and in situ hybridization. ED1+ AMs in allogeneic transplants increased by more than 25-fold from Days 1 to 5. In addition, large, peribronchial/perivascular infiltrates developed containing numerous ED1+ cells. Although AMs in normal rat lungs are CD163−, AMs up-regulated CD163 between Days 4 and 5, reaching maximum values on Day 6. Lymphocytes were less numerous than macrophages. About 16% of the AMs and 10% of the peribronchial/perivascular macrophages were in the S-phase of the cell cycle on Day 2 post-transplantation. No differences in the frequency of BrdU+ macrophages were obvious between isografts and allografts. AMs of donor origin increased in number considerably during allograft rejection. In conclusion, the cellular infiltrate in lung allografts is dominated by macrophages, which exhibit an unusual phenotype and a strong capacity for mitotic self-renewal. |
| Databáze: | OpenAIRE |
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