Internucleosomal chromatin degradation in myeloma and B-hybridoma cell cultures
| Autor: | Andrei Volgin, Sokolova Ia, Veronica V. Volgina, Nikolai N. Khodarev, Shishkin Ss, N. V. Makarova |
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| Rok vydání: | 1992 |
| Předmět: |
Programmed cell death
Ca/Mg-dependent endonuclease education Biophysics Apoptosis In Vitro Techniques Biochemistry Mice Endonuclease Structural Biology Tumor Cells Cultured Genetics Animals Viability assay Fragmentation (cell biology) Molecular Biology B-Lymphocytes Hybridomas Cell Death biology Cell growth Cell Cycle Cell Biology Endonucleases Chromatin Nucleosomes Cell biology Enzyme Activation Cell culture biology.protein Hybridoma DNA Damage Plasmacytoma |
| Zdroj: | FEBS Letters. 313:295-299 |
| ISSN: | 0014-5793 |
| Popis: | The activity of Ca/Mg-dependent endonuclease (CME) is strongly inhibited in myeloma X-63.Ag8.653 and B-hybridoma MLC-1c is compared with mouse splenocytes. Nevertheless, pronounced internucleosomal chromatin degradation occurs in both cell lines during long-term cultivation without passing. In isolated cell nuclei of X-63 the activation of CME, which precedes chromatin fragmentation in vivo and loss of cell viability, is revealed. The time—course of CME activation is opposite to cell proliferation and is not accompanied by alterations in enzyme quantity. The results suggest that cell death of X-63 and MLC-1c occurs via apoptosis, and involves the mechanisms controlling the activation and/or interaction of CME with chromatin. |
| Databáze: | OpenAIRE |
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