Pleiotropic Functions of the Chromodomain-Containing Protein Hat-trick During Oogenesis in Drosophila melanogaster
| Autor: | Mousumi Mutsuddi, Maimuna Sali Paul, D. Dutta, Ashim Mukherjee, Dipti Verma, Ankita Singh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
double-strand break Heterochromatin QH426-470 Biology Investigations Nurse cell Chromodomain 03 medical and health sciences Karyosome formation Gene Knockout Techniques 0302 clinical medicine Genetics Endoreduplication Animals Drosophila Proteins DNA Breaks Double-Stranded Gurken Molecular Biology Genetics (clinical) oogenesis fungi chromodomain Gene Expression Regulation Developmental Cell Cycle Checkpoints Miosis Cell biology Protein Transport 030104 developmental biology Drosophila melanogaster Germ Cells Phenotype germline mosaic Mutation Oocytes Heterochromatin protein 1 Karyosome 030217 neurology & neurosurgery Drosophila Protein hat-trick |
| Zdroj: | G3: Genes|Genomes|Genetics G3: Genes, Genomes, Genetics, Vol 8, Iss 3, Pp 1067-1077 (2018) |
| ISSN: | 2160-1836 |
| Popis: | Chromatin-remodeling proteins have a profound role in the transcriptional regulation of gene expression during development. Here, we have shown that the chromodomain-containing protein Hat-trick is predominantly expressed within the oocyte nucleus, specifically within the heterochromatinized karyosome, and that a mild expression is observed in follicle cells. Colocalization of Hat-trick with Heterochromatin Protein 1 and synaptonemal complex component C(3)G along with the diffused karyosome after hat-trick downregulation shows the role of this protein in heterochromatin clustering and karyosome maintenance. Germline mosaic analysis reveals that hat-trick is required for maintaining the dorso-ventral patterning of eggs by regulating the expression of Gurken. The increased incidence of double-strand breaks (DSBs), delayed DSB repair, defects in karyosome formation, altered Vasa mobility, and, consequently, misexpression and altered localization of Gurken in hat-trick mutant egg chambers clearly suggest a putative involvement of Hat-trick in the early stages of oogenesis. In addition, based on phenotypic observations in hat-trick mutant egg chambers, we speculate a substantial role of hat-trick in cystoblast proliferation, oocyte determination, nurse cell endoreplication, germ cell positioning, cyst encapsulation, and nurse cell migration. Our results demonstrate that hat-trick has profound pleiotropic functions during oogenesis in Drosophila melanogaster. |
| Databáze: | OpenAIRE |
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