A novel cryptic peptide derived from the rat neuropeptide FF precursor reverses antinociception and conditioned place preference induced by morphine
| Autor: | Agnieszka Pachuta, Tomasz Dylag, Hana Raoof, Jerzy Silberring, Jolanta Kotlinska |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Physiology Molecular Sequence Data Neuropeptide Peptide Biochemistry Cellular and Molecular Neuroscience Endocrinology Internal medicine medicine Animals pain Neuropeptide FF Amino Acid Sequence Rats Wistar Peptide sequence chemistry.chemical_classification Morphine Sequence Homology Amino Acid Nociceptors morphine conditioned place preference Conditioned place preference NPFF precursor (85-99) Amino acid Rats Nociception chemistry Conditioning Operant processing Peptides Oligopeptides medicine.drug |
| Zdroj: | Peptides. 29(3) |
| ISSN: | 0196-9781 |
| Popis: | Neuropeptide FF (NPFF) precursors from different species contain at least three known neuropeptides, i.e. FF (FLFQPQRF-NH(2)), AF (AGEGLSSPFWSLAAPQR-NH(2)) and SF (SLAAPQRF-NH(2)). We demonstrate that the rat NPFF precursor contains another bioactive sequence, NAWGPWSKEQLSPQA, spanning between positions 85 and 99. Synthetic NPFF precursor (85-99) (10 and 20 nmol, i.c.v.) blocked the expression of conditioned place preference induced by morphine (5 mg/kg, s.c.). This peptide alone (10 and 20 nmol, i.c.v.) had no influence on the baseline latency of a nociceptive reaction but reversed the antinociceptive activity of morphine (5 mg/kg, s.c.) in the tail-immersion test in rats. These data suggest the existence of a novel bioactive cryptic peptide within an already known NPFF precursor. |
| Databáze: | OpenAIRE |
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