Influence of nociceptin(1-17) fragments and its tyrosine-substituted derivative on morphine-withdrawal signs in rats
| Autor: | Sylwia Talarek, Piotr Rafalski, Jerzy Silberring, Tomasz Dylag, Jolanta Kotlinska, Maria Kosior |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Narcotic Antagonists Vasodilator Agents NOP Motor Activity Naloxone Hydrochloride Cellular and Molecular Neuroscience Endocrinology In vivo Internal medicine morphine-withdrawal syndrome nociceptin medicine Animals rat Rats Wistar Receptor Opioid peptide Morphine Naloxone Endocrine and Autonomic Systems Chemistry General Medicine Peptide Fragments Rats Substance Withdrawal Syndrome Nociceptin receptor Nociception Opioid Peptides morphine dependence Neurology Receptors Opioid Tyrosine Morphine Dependence metabolism medicine.drug |
| Zdroj: | Neuropeptides. 38:277-282 |
| ISSN: | 0143-4179 |
| DOI: | 10.1016/j.npep.2004.05.001 |
| Popis: | Our previous studies demonstrated that endogenous ligand of nociceptin (NOP) receptor, nociceptin(1-17) (also known as orphanin FQ), inhibits morphine-withdrawal syndrome measured as wet dog shakes in rats [Life Sci. 66 (2000) PL119]. This peptide is metabolized in the spinal cord, both in vitro and in vivo, to shorter fragments, including nociceptin(1-11) and nociceptin(1-6). These fragments, formed after cleavage by endogenous peptidase, are behaviorally active and modulate nociception in a bi-phasic process [Peptides 20 (1999) 239]. As these peptides induced transient naloxone-reversible analgesia in behavioral tests [Peptides 20 (1999) 239], in the present study we tested the influence of nociceptin(1-11) (10 and 20 microg) and nociceptin(1-6) (10, 20 and 40 microg) on the morphine-withdrawal syndrome in rats. Furthermore, the modified fragment of nociceptin(1-6) with an opioid-message domain achieved by replacement of Phe1 with Tyr was tested. Morphine-withdrawal syndrome was precipitated by the i.p. injection of naloxone hydrochloride (2 mg/kg), 72 h after implantation of morphine pellets. The wet-dog shakes were chosen for statistical analyses of the abstinence signs. The results show that nociceptin(1-11) and (1-6) attenuate this morphine-withdrawal symptom. The replacement of Phe1 with Tyr in nociceptin(1-6) fragment did not potentiate the influence of nociceptin(1-6) on wet dog shakes precipitated by naloxone in morphine-dependent rats. |
| Databáze: | OpenAIRE |
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