Hypergravity Modifies the Signal Transduction of Ionizing Radiation through p53
| Autor: | Kumio Okaichi, Takeo Ohnishi, Aya Usui, Yutaka Okumura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Ionizing radiation p53 Time Factors Health Toxicology and Mutagenesis Hypergravity Biology Signal transduction Western blot Cyclins Proto-Oncogene Proteins Tumor Cells Cultured medicine Humans Radiology Nuclear Medicine and imaging Irradiation bcl-2-Associated X Protein Radiation medicine.diagnostic_test Glioblastoma cell line Molecular biology Proto-Oncogene Proteins c-bcl-2 Biophysics Tumor Suppressor Protein p53 |
| Zdroj: | Journal of radiation research. 43:261-264 |
| ISSN: | 0449-3060 |
| Popis: | To determine the possible effect of hypergravity to modify the signal transduction of ionizing radiation, we analyzed the accumulation of p53 and the expression of p53-dependent genes, Waf-1 and Bax, using the western blot analysis. Hypergravity (20 × g) induced the accumulation of p53 in the human glioblastoma cell line A172 after 3 h of incubation. Low-dose (0.5 Gy) irradiation to the cells accumulated p53 1.5 h after irradiation, and induced Waf-1 and Bax. Under the condition of hypergravity (20 × g), the peak of p53 accumulation was shifted from 1.5 h to 3 h after irradiation, and the inductions of Waf-1 and Bax were suppressed entirely. These results indicate that hypergravity modifies the signal transduction of ionizing radiation through p53 in the cells. Journal of radiation research. 2002, 43(Supplement), p.261-264 |
| Databáze: | OpenAIRE |
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