Two Overlapping Domains of a Lyssavirus Matrix Protein That Acts on Different Cell Death Pathways
| Autor: | Alireza Gholami, Shahul Mouhamad, Florence Larrous, Hervé Bourhy, Jérôme Estaquier |
|---|---|
| Přispěvatelé: | Lassailly-Bondaz, Anne, Dynamique des Lyssavirus et Adaptation à l'Hôte (DyLAH), Institut Pasteur [Paris] (IP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Pasteur [Paris] |
| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular MESH: Lyssavirus Apoptosis MESH: Amino Acid Sequence MESH: Base Sequence TNF-Related Apoptosis-Inducing Ligand MESH: Recombinant Proteins MESH: Dogs MESH: Protein Structure Tertiary Protein structure Models Site-Directed MESH: Animals Viral MESH: Peptide Fragments Peptide sequence [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology chemistry.chemical_classification 0303 health sciences 030302 biochemistry & molecular biology MESH: Amino Acid Substitution Recombinant Proteins Virus-Cell Interactions MESH: Rabies virus 3. Good health Amino acid MESH: Mutagenesis Site-Directed Host-Pathogen Interactions [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: TNF-Related Apoptosis-Inducing Ligand MESH: Models Molecular MESH: DNA Primers Protein Structure Programmed cell death Molecular Sequence Data Immunology Biology Microbiology Electron Transport Complex IV Viral Matrix Proteins 03 medical and health sciences Dogs MESH: Electron Transport Complex IV Virology Animals Humans Cytochrome c oxidase Amino Acid Sequence Lyssavirus DNA Primers 030304 developmental biology MESH: Viral Matrix Proteins MESH: Humans MESH: Molecular Sequence Data Viral matrix protein Base Sequence MESH: Apoptosis MESH: Host-Pathogen Interactions Molecular DNA biology.organism_classification Molecular biology Peptide Fragments Protein Structure Tertiary MESH: DNA Viral Amino Acid Substitution chemistry Mutagenesis Rabies virus Insect Science DNA Viral MESH: HeLa Cells Mutagenesis Site-Directed biology.protein Tertiary HeLa Cells |
| Zdroj: | Journal of Virology Journal of Virology, 2010, 84 (19), pp.9897-906. ⟨10.1128/JVI.00761-10⟩ Journal of Virology, American Society for Microbiology, 2010, 84 (19), pp.9897-906. ⟨10.1128/JVI.00761-10⟩ |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.00761-10 |
| Popis: | The lyssavirus matrix (M) protein induces apoptosis. The regions of the M protein that are essential for triggering cell death pathways are not yet clearly defined. We therefore compared the M proteins from two viruses that have contrasting characteristics in terms of cellular apoptosis: a genotype 3 lyssavirus, Mokola virus (MOK), and a genotype 1 rabies virus isolated from a dog from Thailand (THA). We identified a 20-amino-acid fragment (corresponding to positions 67 to 86) that retained the cell death activities of the full-length M protein from MOK via both the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and inhibition of cytochromecoxidase (CcO) activity. We found that the amino acids at positions 77 and 81 have an essential role in triggering these two cell death pathways. Directed mutagenesis demonstrated that the amino acid at position 77 affects CcO activity, whereas the amino acid at position 81 affects TRAIL-dependent apoptosis. Mutations in the full-length M protein that compromised induction of either of these two pathways resulted in delayed apoptosis compared with the time to apoptosis for the nonmutated control. |
| Databáze: | OpenAIRE |
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