Determination of limiting mobilities and dissociation constants of 21 amino acids by capillary zone electrophoresis at very low pH
| Autor: | Alena Novotná, Katerina Vceláková, Iva Zusková, Bohuslav Gaš |
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| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Chromatography Chemistry Clinical Biochemistry Analytical chemistry Electrophoresis Capillary Protonation Cell Biology General Medicine Electrolyte Hydrogen-Ion Concentration Conductivity Biochemistry Analytical Chemistry Amino acid Dissociation constant Electrophoresis Capillary electrophoresis Ionic strength Thermodynamics Amino Acids |
| Zdroj: | Journal of Chromatography B. 841:129-134 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2006.03.015 |
| Popis: | The dependence of the effective electrophoretic mobility on pH of the background electrolyte was experimentally determined by capillary zone electrophoresis (CZE) for cationic forms of amino acids. The pH of the background electrolytes was in the highly acidic range, 1.6-2.6 pH units, to ensure a high degree of protonation of the amino acids. Poly(vinyl alcohol) was added to the background electrolytes to avoid possible adsorption of the analytes at the inner capillary wall. Non-linear regression of the experimental data was applied to obtain the parameters of the relevant regression functions--the actual mobilities and mixed dissociation constants corresponding to the actual ionic strength. The extended Onsager and Debye-Hückel law was used to calculate the limiting mobilities and thermodynamic dissociation constants. The comparison of the experimental electropherogram with the computer prediction by PeakMaster using the determined data is presented for the selected sample of amino acids. |
| Databáze: | OpenAIRE |
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