Mitochondrial plasmids of the pCp family are spread worldwide in Cryphonectria parasitica populations
| Autor: | Djaouida Rekab, Emanuela Gobbi, Romano Locci |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Genetics
education.field_of_study biology fungi Population Plant Science biology.organism_classification Plasmid Molecular evolution Phylogenetics Multiplex polymerase chain reaction Genetic variability Cryphonectria Restriction fragment length polymorphism education Ecology Evolution Behavior and Systematics Biotechnology |
| Zdroj: | Mycological Research. 106:1408-1416 |
| ISSN: | 0953-7562 |
| DOI: | 10.1017/s0953756202006810 |
| Popis: | A worldwide collection of strains of Cryphonectria parasitica was examined to draw a precise picture of the incidence and diversity of mitochondrial plasmids related to the plasmid pUG1. Amplification by specific PCR of 199 strains showed the presence of pUG1-like plasmids in 22% of the populations examined. The entire plasmid molecules were amplified by multiplex PCR and the products showed different RFLP patterns. The variability was mostly in a non-coding region of the molecule that has been sequenced in some representative strains, enabling the molecular evolution of the molecule to be elucidated. The data show that mitochondrial plasmids of C. parasitica comprise an almost homogeneous family (designated pCp) that can be divided into two clusters based on the presence/absence respectively of a 60 nucleotide region in North American and European plasmids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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