Infrared spectroscopy of synovial fluid as a potential screening approach for the diagnosis of naturally occurring canine osteoarthritis associated with cranial cruciate ligament rupture
| Autor: | Romain Béraud, Sarah Malek, Christopher B. Riley, Federico Marini, Glenda M. Wright, Mark C. Rochat |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty OsteoarthritisBiomarkerInfrared spectroscopyKneeDogSynovial fluid Screening test 040301 veterinary sciences Osteoarthritis Diseases of the musculoskeletal system 0403 veterinary science Cruciate ligament 03 medical and health sciences 0302 clinical medicine medicine Dog Synovial fluid Knee Absorbance spectra Infrared spectroscopy 030203 arthritis & rheumatology business.industry 04 agricultural and veterinary sciences Biomarker medicine.disease medicine.anatomical_structure RC925-935 Biomarker (medicine) business |
| Zdroj: | Osteoarthritis and Cartilage Open, Vol 2, Iss 4, Pp 100120-(2020) |
| ISSN: | 2665-9131 |
| Popis: | Objective: To evaluate infrared (IR) spectroscopy of synovial fluid (SF) as tool to differentiate between knees of dogs with naturally occurring OA associated with cranial cruciate ligament rupture (CrCLR) and controls. Method: 104 adult dogs with CrCLR (affected group) and 50 adult control dogs were recruited in a prospective observational study. Synovial fluid (SF) samples were collected preoperatively from dogs with CrCLR and from a subset of these at 4-, and 12-week post-surgery. Knee samples were collected bilaterally once from control dogs. Dried synovial fluid films were made, and IR absorbance spectra acquired. After preprocessing, partial least squares discriminant analysis (PLS-DA) and ANOVA-simultaneous component analysis (ASCA) were used to evaluate group and temporal differences, and to develop predictive models. Results: There were statistically significant spectral differences between the SF of OA affected and control dogs at all three time-points (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|