In vivo magnetic resonance microscopy of Drosophilae at 9.4 T
| Autor: | Joël Mispelter, Frédéric Szeremeta, Sandra Même, Jean-Claude Beloeil, Daniel Locker, Martine Decoville, Nicolas Joudiou, Fanny Louat |
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| Přispěvatelé: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Image generation
Biomedical Engineering Biophysics Biology Sensitivity and Specificity 03 medical and health sciences Preclinical research 0302 clinical medicine Nuclear magnetic resonance In vivo Small animal Animals Radiology Nuclear Medicine and imaging Whole Body Imaging [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology 0303 health sciences Microscopy Magnetic resonance microscopy Reproducibility of Results Muscle degeneration Equipment Design Magnetic field gradient Image Enhancement Magnetic Resonance Imaging Equipment Failure Analysis Drosophila melanogaster Electromagnetic coil 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Magnetic Resonance Imaging Magnetic Resonance Imaging, Elsevier, 2013, 31 (1), pp.109-19. ⟨10.1016/j.mri.2012.06.019⟩ |
| ISSN: | 0730-725X |
| DOI: | 10.1016/j.mri.2012.06.019⟩ |
| Popis: | International audience; In preclinical research, genetic studies have made considerable progress as a result of the development of transgenic animal models of human diseases. Consequently, there is now a need for higher resolution MRI to provide finer details for studies of small animals (rats, mice) or very small animals (insects). One way to address this issue is to work with high-magnetic-field spectrometers (dedicated to small animal imaging) with strong magnetic field gradients. It is also necessary to develop a complete methodology (transmit/receive coil, pulse sequence, fixing system, air supply, anesthesia capabilities, etc.). In this study, we developed noninvasive protocols, both in vitro and in vivo (from coil construction to image generation), for drosophila MRI at 9.4 T. The 10 10 80-μm resolution makes it possible to visualize whole drosophila (head, thorax, abdomen) and internal organs (ovaries, longitudinal and transverse muscles, bowel, proboscis, antennae and optical lobes). We also provide some results obtained with a Drosophila model of muscle degeneration. This opens the way for new applications of structural genetic modification studies using MRI of drosophila. |
| Databáze: | OpenAIRE |
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