Histological effects and pharmacokinetics of lipopolysaccharide derived from Porphyromonas gingivalis on rat maxilla and liver concerning with progression into non‐alcoholic steatohepatitis
| Autor: | Yukihiro Numabe, Ryutaro Kuraji, Hiroshi Ito, Toshiyuki Toen, Shuichi Hashimoto, Miyako Fujita, Tetsuya Fukada |
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| Rok vydání: | 2018 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Lipopolysaccharide Gingiva Inflammation 03 medical and health sciences chemistry.chemical_compound Ballooning degeneration 0302 clinical medicine Non-alcoholic Fatty Liver Disease Internal medicine Maxilla medicine Animals Tissue Distribution Porphyromonas gingivalis Periodontitis biology business.industry Histology 030206 dentistry medicine.disease biology.organism_classification Rats medicine.anatomical_structure Endocrinology chemistry Disease Progression Periodontics lipids (amino acids peptides and proteins) 030211 gastroenterology & hepatology Hard palate Steatohepatitis medicine.symptom business |
| Zdroj: | Journal of Periodontology. 89:1101-1111 |
| ISSN: | 1943-3670 0022-3492 |
| Popis: | Background Non-alcoholic steatohepatitis (NASH) is one of the chronic liver diseases that can develop into hepatocirrhosis. The purpose of the present study was to investigate the impact of lipopolysaccharide (LPS) from Porphyromonas gingivalis (P. gingivalis) on NASH onset, and to determine the biodistribution of double-radiolabeled LPS (R-LPS) biosynthesized in P. gingivalis. Methods Rats fed a basal diet (BD) or a high-fat diet (HD) were injected with P. gingivalis-LPS or R-LPS into the palatine gingiva around the right maxillary first molar, and were classified into the following 4 groups: BD/LPS (-), BD/LPS (+), HD/LPS (-), and HD/LPS (+) or 2 groups: BD/R-LPS and HD/R-LPS. Results Inflammation in the gingiva of the LPS (+) groups progressed significantly more than the LPS (-) groups. Furthermore, in the HD/LPS (+) liver, histologic analysis confirmed the presence of NASH, characterized by large fat droplets, ballooning degeneration, and infiltration of inflammatory cells. When 3 H, 14 C-R-LPS was injected into the palatine gingiva, radioactivity in the right palatal mucosa of HD/R-LPS rats was the highest in comparison with other regions and was significantly elevated after 24 hours compared to BD/R-LPS rats. Autoradiographic analysis of the maxilla showed distributions from the palatal mucosa to the hard palate and the interdental region. Radioactivity in organs of both BD/R-LPS and HD/R-LPS rats were mostly localized to the liver even after 24 hours. Conclusion The present study suggests that the transfer of P. gingivalis-LPS from the oral cavity to the liver plays an important role in disease exacerbation of NASH. |
| Databáze: | OpenAIRE |
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