Dietary magnesium restriction affects hematopoiesis and triggers neutrophilia by increasing STAT-3 expression and G-CSF production

Autor: Araceli Aparecida Hastreiter, Edson Naoto Makiyama, Agatha Brida de Sousa Castelhano, Fabiana da Silva Lima, Ricardo Ambrósio Fock
Rok vydání: 2021
Předmět:
Male
STAT3 Transcription Factor
0301 basic medicine
medicine.medical_specialty
Neutrophils
medicine.medical_treatment
Bone Marrow Cells
030209 endocrinology & metabolism
Context (language use)
Critical Care and Intensive Care Medicine
Granulopoiesis
Leukocyte Count
Mice
03 medical and health sciences
0302 clinical medicine
Internal medicine
Granulocyte Colony-Stimulating Factor
medicine
Animals
Magnesium
STAT3
030109 nutrition & dietetics
Nutrition and Dietetics
biology
MAGNÉSIO
business.industry
Cell Cycle
Hematopoietic Stem Cells
Neutrophilia
Diet
Granulocyte colony-stimulating factor
Mice
Inbred C57BL
Haematopoiesis
Cytokine
Endocrinology
medicine.anatomical_structure
CCAAT-Enhancer-Binding Proteins
biology.protein
Calcium
Leukopoiesis
Bone marrow
medicine.symptom
business
Magnesium Deficiency
Granulocytes
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0261-5614
Popis: Summary Background & aims Magnesium (Mg2+) is able to modulate the differentiation and proliferation of cells. Mg2+ restriction can trigger neutrophilia, but the processes that result in this change have yet to be investigated and are not fully understood. Hematopoiesis is a complex process that is regulated by many factors, including cytokines and growth factors, and is strongly influenced by nutrient availability. In this context, our objective was to investigate the impact of the short-term restriction of dietary Mg2+ on bone marrow hematopoietic and peripheral blood cells, especially in processes related to granulocyte differentiation and proliferation. Methods Male C57BL/6 mice were fed a Mg2+ restricted diet (50 mg Mg2+/kg diet) for 4 weeks. Cell blood count and bone marrow cell count were evaluated. Bone marrow cells were also characterized by flow cytometry. Gene expression and cytokine production were evaluated, and a colony-forming cell assay related to granulocyte differentiation and proliferation was performed. Results Short-term dietary restriction of Mg2+ resulted in peripheral neutrophilia associated with an increased number of granulocytic precursors in the bone marrow. Additionally, Mg2+ restriction resulted in an increased number of granulocytic colonies formed in vitro. Moreover, the Mg2+ restricted group showed increased expression of CSF3 and CEBPα genes as well as increased production of G-CSF in association with increased expression of STAT3 protein. Conclusion Short-term dietary restriction of Mg2+ induces granulopoiesis by increasing G-CSF production and activating the CEBPα and STAT-3 pathways, resulting in neutrophilia in peripheral blood.
Databáze: OpenAIRE
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