A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
| Autor: | Nuno Bandeira, Fabricio K Marchini, Felipe da Veiga Leprevost, Silvio Sanches Veiga, Dilza Trevisan-Silva, Aline Viana Bednaski, Adrian Guthals, Juliana de Saldanha da Gama Fischer, Andrea Senff-Ribeiro, Valmir C. Barbosa, Paulo C. Carvalho |
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| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Statistics and Probability Data Descriptor Proteome Spider Venoms medicine.medical_treatment Venom Library and Information Sciences Mass spectrometry Mass Spectrometry Education 03 medical and health sciences medicine Protein analysis Animals Protease Chymotrypsin 030102 biochemistry & molecular biology biology Spiders Trypsin Molecular biology Computer Science Applications 030104 developmental biology Biochemistry biology.protein Statistics Probability and Uncertainty Entomology Peptide Hydrolases Information Systems medicine.drug |
| Zdroj: | Scientific Data |
| ISSN: | 2052-4463 |
| Popis: | Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523. |
| Databáze: | OpenAIRE |
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