TCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network
| Autor: | Luís Korrodi-Gregório, Joana Silva, Odete A. B. da Cruz e Silva, Joana Abrantes, Pedro J. Esteves, Margarida Fardilha, Edgar F. da Cruz e Silva, Maria João Freitas, Sara L. C. Esteves, Ann-Kristin Brauns, Georg H. Lüers, Sandra Vieira |
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| Přispěvatelé: | Universitat de Barcelona |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
QH301-705.5
Science Phosphatase Dynein Acrosome reaction Tctex1d4 Microtúbuls Flagellum Biology Microtubules General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Fosforilació 0302 clinical medicine Microtubule Semen Testis Proteïnes citosquelètiques Phosphorylation Biology (General) Acrosome 030304 developmental biology Genetics 0303 health sciences Microtubule organizing center PP1 Sperm Cell biology Cytoskeletal proteins Testicle General Agricultural and Biological Sciences 030217 neurology & neurosurgery Binding domain Research Article |
| Zdroj: | Biology Open, Vol 2, Iss 5, Pp 453-465 (2013) Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona Biology Open |
| ISSN: | 2046-6390 |
| Popis: | Summary Reversible phosphorylation plays an important role as a mechanism of intracellular control in eukaryotes. PPP1, a major eukaryotic Ser/Thr-protein phosphatase, acquires its specificity by interacting with different protein regulators, also known as PPP1 interacting proteins (PIPs). In the present work we characterized a physiologically relevant PIP in testis. Using a yeast two-hybrid screen with a human testis cDNA library, we identified a novel PIP of PPP1CC2 isoform, the T-complex testis expressed protein 1 domain containing 4 (TCTEX1D4) that has recently been described as a Tctex1 dynein light chain family member. The overlay assays confirm that TCTEX1D4 interacts with the different spliced isoforms of PPP1CC. Also, the binding domain occurs in the N-terminus, where a consensus PPP1 binding motif (PPP1BM) RVSF is present. The distribution of TCTEX1D4 in testis suggests its involvement in distinct functions, such as TGFβ signaling at the blood–testis barrier and acrosome cap formation. Immunofluorescence in human ejaculated sperm shows that TCTEX1D4 is present in the flagellum and in the acrosome region of the head. Moreover, TCTEX1D4 and PPP1 co-localize in the microtubule organizing center (MTOC) and microtubules in cell cultures. Importantly, the TCTEX1D4 PPP1BM seems to be relevant for complex formation, for PPP1 retention in the MTOC and movement along microtubules. These novel results open new avenues to possible roles of this dynein, together with PPP1. In essence TCTEX1D4/PPP1C complex appears to be involved in microtubule dynamics, sperm motility, acrosome reaction and in the regulation of the blood–testis barrier. |
| Databáze: | OpenAIRE |
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