Use of Thrombodynamics for revealing the participation of platelet, erythrocyte, endothelial, and monocyte microparticles in coagulation activation and propagation

Autor: O. A. Antonova, A. V. Mazurov, Karolina Losenkova, Elena N. Lipets, Fazoil I. Ataullakhanov, O. N. Shustova
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Erythrocytes
Light
Physiology
030204 cardiovascular system & hematology
Biochemistry
Monocytes
Scattering
chemistry.chemical_compound
White Blood Cells
0302 clinical medicine
Thrombodynamics test
Animal Cells
Cell-Derived Microparticles
Red Blood Cells
Medicine and Health Sciences
Platelet
Calcimycin
Multidisciplinary
Chemistry
Physics
Electromagnetic Radiation
Thrombin
Phosphatidylserine
Flow Cytometry
Body Fluids
medicine.anatomical_structure
Blood
Coagulation
Physical Sciences
Medicine
Anatomy
Cellular Types
Cellular Structures and Organelles
medicine.drug
Research Article
Platelets
Blood Platelets
Science
Immune Cells
Immunology
Blood Plasma
03 medical and health sciences
Tissue factor
medicine
Human Umbilical Vein Endothelial Cells
Humans
Vesicles
Plasma Volume
Blood Coagulation
Blood Cells
Monocyte
Light Scattering
Biology and Life Sciences
Proteins
Endothelial Cells
Thrombosis
Cell Biology
Peptide Fragments
030104 developmental biology
Hemostasis
Biophysics
Calcium
Zdroj: PLoS ONE
PLoS ONE, Vol 15, Iss 5, p e0227932 (2020)
ISSN: 1932-6203
Popis: Background and objective For many pathological states, microparticles are supposed to be one of the causes of hypercoagulation. Although there are some indirect data about microparticles participation in coagulation activation and propagation, the integral hemostasis test Thrombodynamics allows to measure micropaticles participation in these two coagulation phases directly. Demonstrates microparticles participation in coagulation activation by influence on the appearance of coagulation centres in the plasma volume and the rate of clot growth from the surface with immobilized tissue factor.Methods: Microparticles were obtained from platelets and erythrocytes by stimulation with thrombin receptor-activating peptide (SFLLRN) and calcium ionophore (A23187), respectively, from monocytes, endothelial HUVEC culture and monocytic THP cell culture by stimulation with lipopolysaccharides. Microparticles were counted by flow cytometry and titrated in microparticle-depleted normal plasma in the Thrombodynamics test. Results Monocyte microparticles induced the appearance of clotting centres through the TF pathway at concentrations approximately 100-fold lower than platelet and erythrocyte microparticles, which activated plasma by the contact pathway. For endothelial microparticles, both activation pathways were essential, and their activity was intermediate. Monocyte microparticles induced plasma clotting by the appearance of hundreds of clots with an extremely slow growth rate, while erythrocyte microparticles induced the appearance of a few clots with a growth rate similar to that from surface covered with high-density tissue factor. Patterns of clotting induced by platelet and endothelial microparticles were intermediate. Platelet, erythrocyte and endothelial microparticles impacts on the rate of clot growth from the surface with tissue factor did not differ significantly within the 0-200·103/ul range of microparticles concentrations. However, at concentrations greater than 500·103/ul, erythrocyte microparticles increased the stationary clot growth rate to significantly higher levels than do platelet microparticles or artificial phospholipid vesicles consisting of phosphatidylcholine and phosphatidylserine. Conclusion Microparticles of different origins demonstrated qualitatively different characteristics related to coagulation activation and propagation.
Databáze: OpenAIRE
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