Antimicrobial photodynamic therapy (aPDT) with curcumin controls intradermal infection by Staphylococcus aureus in mice with type 1 diabetes mellitus: a pilot study
| Autor: | Denisar Palmito dos Santos, Israel S. Ribeiro, Dirceu Joaquim Costa, Barbara Porto Cipriano, Emely Soares Silva, Caroline Vieira Gonçalves, Telma de Jesus Soares, Igor Pereira Ribeiro Muniz, Thiago Macêdo Lopes Correia, Tatyana Chagas Moura, Leandro Martins de Freitas, Robson Amaro Augusto da Silva, Nathalia Rosa Silva, Maria Poliana Leite Galantini |
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| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Curcumin medicine.medical_treatment Biophysics Photodynamic therapy Pilot Projects Pharmacology medicine.disease_cause Streptozocin chemistry.chemical_compound Mice medicine Animals Radiology Nuclear Medicine and imaging Saline Lymph node Type 1 diabetes Radiation Radiological and Ultrasound Technology business.industry Skin Diseases Bacterial Staphylococcal Infections medicine.disease Antimicrobial Mice Inbred C57BL medicine.anatomical_structure Diabetes Mellitus Type 1 chemistry Photochemotherapy Staphylococcus aureus Inflammation Mediators business Ex vivo Biomarkers |
| Zdroj: | Journal of photochemistry and photobiology. B, Biology. 224 |
| ISSN: | 1873-2682 |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens that cause infections in diabetic individuals. In this paper, we report the outcomes of our investigation on the intradermal application of antimicrobial photodynamic therapy (PDT) with curcumin in an infection induced by MRSA ATCC 43300 strain in the ear of mice with Type 1 Diabetes Mellitus (T1DM). A solution containing 100 μg of curcumin was photoactivated ex vivo with a LED light (450 nm) delivering a fluency of 13.5 J/cm3. This solution was administered in the ear intradermally, at the same inoculum site as the MRSA ATCC 43300 strain (PDT Group). This study also included the use of two control groups (both infected): One was treated with saline and the other was treated with non-photoactivated curcumin. The animals were euthanized 24 h after these treatments and samples of draining lymph node and treated ear were collected for examination. The PDT group showed lower bacterial load in the draining lymph node when compared to the saline and curcumin groups (p-value |
| Databáze: | OpenAIRE |
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