Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration
| Autor: | Linda R Wang Lam, Stephen Romas, Jack G. Caton, Xinping Zhang, Ravi S. Misra, Zhuang Zhou, Kevin Schilling |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Periodontal ligament stem cells Periodontal Ligament 0206 medical engineering Nanofibers ALIZARIN RED 02 engineering and technology Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Osteogenesis Enamel matrix derivative Bovine serum albumin Bone regeneration General Dentistry Cell Proliferation biology Cell Differentiation 030206 dentistry 020601 biomedical engineering Membrane chemistry Nanofiber Polycaprolactone Guided Tissue Regeneration Periodontal Ceramics and Composites biology.protein Biomedical engineering |
| Zdroj: | Dent Mater J |
| ISSN: | 1881-1361 0287-4547 |
| Popis: | The osteogenic effect of a composite electrospun core-shell nanofiber membrane encapsulated with Emdogain® (EMD) was evaluated. The membrane was developed through coaxial electrospinning using polycaprolactone as the shell and polyethylene glycol as the core. The effects of the membrane on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) were examined using Alizarin Red S staining and qRT-PCR. Characterization of the nanofiber membrane demonstrated core-shell morphology with a mean diameter of ~1 µm. Examination of the release of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) from core-shell nanofibers over a 22-day period showed improved release profile of encapsulated proteins as compared to solid nanofibers. When cultured on EMD-containing core-shell nanofibers, PDLSCs showed significantly improved osteogenic differentiation with increased Alizarin Red S staining and enhanced osteogenic gene expression, namely OCN, RUNX2, ALP, and OPN. Core-shell nanofiber membranes may improve outcomes in periodontal regenerative therapy through simultaneous mechanical barrier and controlled drug delivery function. |
| Databáze: | OpenAIRE |
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