Phagocytosis and LPS-stimulated production of cytokines and prostaglandin E2 is different in Kupffer cells isolated from the periportal or perivenous liver region
| Autor: | P Ylipaasto, L Eerola, K O Lindros, I Bykov |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male Pathology medicine.medical_specialty Lipopolysaccharide Liver cytology Kupffer Cells medicine.medical_treatment Phagocytosis Cell Separation Biology Nitric Oxide Dinoprostone chemistry.chemical_compound medicine Escherichia coli Animals Prostaglandin E2 Rats Wistar Cells Cultured Microscopy Confocal Tumor Necrosis Factor-alpha Kupffer cell Zymosan Gastroenterology Molecular biology Rats medicine.anatomical_structure Cytokine chemistry Liver Cytokines Tumor necrosis factor alpha medicine.drug Interleukin-1 |
| Zdroj: | Scandinavian journal of gastroenterology. 38(12) |
| ISSN: | 0036-5521 |
| Popis: | Kupffer cells can release pro-inflammatory mediators and contribute to damage, which often appears in a zonated fashion.To assess position-associated functional differences, functions of intact Kupffer cells isolated from either the periportal or perivenous acinar region of rat liver were compared.Kupffer cells from the periportal region phagocytosed 2-3 times more FITC-labelled zymosan particles than corresponding perivenous cells, as determined by confocal microscopy and fluorescence assay. Periportal cells also produced more TNF-alpha and IL-1beta, but less NO and PGE2, compared to perivenous cells and the stimulation by addition of lipopolysaccharides (LPS) was moderate. In contrast, after overnight culture LPS dramatically increased TNF-alpha release and significantly more so in perivenous Kupffer cells (26-fold) than in periportal cells (11-fold).Our study suggests that periportal Kupffer cells are responsible for a major part of phagocytosis by the liver. The stronger LPS response of recovered perivenous Kupffer cells suggests a dominant role of these cells in pro-inflammatory events that ultimately may contribute to development of damage in this region. |
| Databáze: | OpenAIRE |
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