Effects of the phosphatidylinositol 3-kinase inhibitor in a mouse model of retinal neovascularization
| Autor: | Jian-Qiang Dong, He Zou, Zheng Yan, Xiaoxin Li, Wen-Zhen Yu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty genetic structures Cell Survival Morpholines Cell Biology In Vitro Techniques Retinal Neovascularization Retina Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Cell Movement Ischemia Internal medicine medicine Animals LY294002 Phosphatidylinositol Enzyme Inhibitors Cells Cultured Phosphoinositide-3 Kinase Inhibitors Apyrase Retinal Vessels Inner limiting membrane General Medicine Anatomy Macaca mulatta eye diseases Sensory Systems Endothelial stem cell Vascular endothelial growth factor Mice Inbred C57BL Ophthalmology medicine.anatomical_structure Endocrinology chemistry Animals Newborn Chromones sense organs Perfusion |
| Zdroj: | Ophthalmic research. 40(1) |
| ISSN: | 1423-0259 |
| Popis: | Purpose: To determine the effectsof LY294002, a phosphatidylinositol 3-kinase inhibitor, on suppressing experimental retinal neovascularization in an animal model of ischemic retinopathy. Methods: The effect of LY294002 on the survival of RF/6A cells stimulated by vascular endothelial growth factor (VEGF) was investigated colorimetrically. The inhibitory activity of LY294002 on the migration of cells stimulated with VEGF was measured by cell counting. C57BL/6N mice at postnatal day (P) 7 were exposed to 75 ± 2% oxygen for 5 days (P7–P11) and then returned to room air for 5 days (P12–P17) to induce retinal neovascularization. Beginning on P12, mice received daily intraperitoneal injections of LY294002 or dimethyl sulfoxide and phosphate-buffered saline (control) through P17. Retinal neovascularization was examined by adenosine diphosphatase staining after 5 days in room air and was quantitated histologically by counting the neovascular endothelial cell nuclei anterior to the inner limiting membrane. Results: LY294002 significantly inhibited VEGF-induced survival and migration. LY294002-treated and control animals demonstrated no perfusion regions in the posterior retina. Retinas from control mice at P17 contained neovascular tufts at the junction between the perfused and nonperfused retina. The tufts contained numerous neovascular nuclei. Retinas from mice treated with LY294002 demonstrated a significant reduction in neovascular cell nuclei compared with control mice. Conclusions: LY294002 significantly inhibits retinal neovascularization in a mouse model of retinal neovascularization. |
| Databáze: | OpenAIRE |
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