A major checkpoint for protein expression in Rhodobacter sphaeroides during heat stress response occurs at the level of translation
| Autor: | Jonas Kretz, Matthew McIntosh, Anne Konzer, Gabriele Klug, Anna Latz, Thorsten Köchling, Sandra Heinen |
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| Rok vydání: | 2021 |
| Předmět: |
Regulation of gene expression
Proteomics biology RNA Sigma Factor Gene Expression Regulation Bacterial Rhodobacter sphaeroides biology.organism_classification Microbiology Cell biology Transcriptome Bacterial Proteins Transcription (biology) Sigma factor Heat shock protein Gene expression Ecology Evolution Behavior and Systematics Heat-Shock Response |
| Zdroj: | Environmental microbiologyReferences. 23(11) |
| ISSN: | 1462-2920 |
| Popis: | Temperature above the physiological optimum is a stress condition frequently faced by bacteria in their natural environments. Here, we were interested in the correlation between levels of RNA and protein under heat stress. Changes in RNA and protein levels were documented in cultures of Rhodobacter sphaeroides using RNA sequencing, quantitative mass spectrometry, western blot analysis, in vivo [35 S] methionine-labelling and plasmid-borne reporter fusions. Changes in the transcriptome were extensive. Strikingly, the proteome remained unchanged except for very few proteins. Examples include a heat shock protein, a DUF1127 protein of unknown function and sigma factor proteins from leaderless transcripts. Insight from this study indicates that R. sphaeroides responds to heat stress by producing a broad range of transcripts while simultaneously preventing translation from nearly all of them, and that this selective production of protein depends on the untranslated region of the transcript. We conclude that measurements of transcript abundance are insufficient to understand gene regulation. Rather, translation can be an important checkpoint for protein expression under certain environmental conditions. Furthermore, during heat shock, regulation at the level of transcription might represent preparation for survival in an unpredictable environment while regulation at translation ensures production of only a few proteins. |
| Databáze: | OpenAIRE |
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