Quantitative analysis of S. mutans, Lactobacillus and Bifidobacterium found in initial and mature plaques in Thai children with early childhood caries
| Autor: | Kemthong Mitrakul, A Jeamset, K Vongsawan, S Chanvitan |
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| Rok vydání: | 2017 |
| Předmět: |
Parents
0301 basic medicine medicine.medical_specialty Plaque index Dental Caries Dental plaque Polymerase Chain Reaction Gastroenterology Oral hygiene White People Microbiology Streptococcus mutans 03 medical and health sciences 0302 clinical medicine Asian People Internal medicine Lactobacillus Humans Medicine Dentistry (miscellaneous) Bifidobacterium biology business.industry 030206 dentistry Oral Hygiene biology.organism_classification medicine.disease stomatognathic diseases 030104 developmental biology Child Preschool Pediatrics Perinatology and Child Health business Quantitative analysis (chemistry) Early childhood caries |
| Zdroj: | European Archives of Paediatric Dentistry. 18:251-261 |
| ISSN: | 1996-9805 1818-6300 |
| Popis: | To quantify Streptococcus mutans, lactobacillus and bifidobacterium in initial and mature plaque collected from children with severe early childhood caries (S-ECC) and caries-free (CF) groups and to analyse the association between these bacteria and caries-related factors in each group. A collection of 120 initial and overnight supra-gingival plaques were collected from Thai children aged 2–5 years-old (S-ECC = 60, CF = 60). Plaque, gingival indices and decayed, missing, filled tooth (dmft) scores were recorded. A questionnaire was used to assess the parents’ attitudes and behaviour regarding the child’s oral hygiene care and diet. After DNA extraction, quantitative real-time polymerase chain reaction (PCR) using fluorescent dye (SYBR green) was performed. Levels of Streptococcus mutans, lactobacillus and bifidobacterium in both initial and mature plaques of S-ECC were significantly higher than those from the caries-free group (p |
| Databáze: | OpenAIRE |
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