Linking differential radiation responses to glioma heterogeneity
| Autor: | Yi-Hong Zhou, Yuanjie Hu, Chao Ke, Charles L. Limoli, Mark E. Linskey, Liping Yu, Anne T. Di Donato, Katherine K. Tran, Yumay Chen, Ping H. Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Ionizing
Oncology and Carcinogenesis Apoptosis Oxidative phosphorylation Biology medicine.disease_cause Radiation Tolerance Oxidative Phosphorylation Colony-Forming Units Assay Rare Diseases Radiation Ionizing Spheroids Cellular glioma Glioma Radioresistance cellular heterogeneity Gene expression Tumor Cells Cultured medicine Humans 2.1 Biological and endogenous factors Glycolysis Aetiology Cell Proliferation Cancer Cultured Radiation redox homeostasis metabolic state Spheroid Flow Cytometry medicine.disease Phenotype Molecular biology Tumor Cells Brain Disorders Brain Cancer Oncology radiosensitivity Cancer research tumorigenicity Cellular Spheroids Energy Metabolism Oxidative stress Research Paper |
| Zdroj: | Oncotarget, vol 5, iss 6 Ke, C; Tran, K; Chen, Y; di Donato, AT; Yu, L; Hu, Y; et al.(2014). Linking differential radiation responses to glioma heterogeneity. Oncotarget, 5(6), 1657-1665. doi: 10.18632/oncotarget.1823. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/045895p6 Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.1823 |
| Popis: | The phenotypic and genetic diversity that define tumor subpopulations within high-grade glioma can lead to therapeutic resistance and tumor recurrence. Given that cranial irradiation is a frontline treatment for malignant glioma, understanding how irradiation selectively effects different cellular subpopulations within these heterogeneous cancers should help identify interventions targeted to better combat this deadly disease. To analyze the radiation response of distinct glioma subpopulations, 2 glioma cells lines (U251, A172) were cultured under conditions that promoted either adherence or non-adherent spheroids. Past work has demonstrated that subpopulations derived from defined culture conditions exhibit differences in karyotype, proliferation, gene expression and tumorigenicity. Spheroid cultures from each of the glioma cell lines were found to be more radiosensitive, which was consistent with higher levels of oxidative stress and lower levels of both oxidative phosphorylation and glycolytic metabolism 1 week following irradiation. In contrast, radioresistant non-spheroid parental cultures showed increased glycolytic activity in response to irradiation, while oxidative phosphorylation was affected to a lesser extent. Overall these data suggest that prolonged radiation-induced oxidative stress can compromise the metabolic state of certain glioma subpopulations thereby altering their sensitivity to an important therapeutic intervention used routinely for the control of glioma. |
| Databáze: | OpenAIRE |
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