The effectiveness of a CpG motif-based adjuvant (CpG ODN 2006) for LHRH immunization
| Autor: | Nada S. Cummings, David M. de Avila, Kenny J. Wells, Valéria A. Conforti, Jerry J. Reeves, Hasan Ülker |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
endocrine system
CpG Oligodeoxynucleotide medicine.drug_class Ovalbumin medicine.medical_treatment Pharmacology Acetates Immunostimulant Antibodies law.invention Mycobacterium Gonadotropin-Releasing Hormone Rats Sprague-Dawley Antigen Adjuvants Immunologic law medicine Escherichia coli Animals Antigens Contraception Immunologic Immunocontraception Methylene Chloride Vaccines General Veterinary General Immunology and Microbiology Chemistry Immunogenicity Ovary Public Health Environmental and Occupational Health Organ Size respiratory system Rats Infectious Diseases Freeze Drying CpG site Biochemistry Oligodeoxyribonucleotides Recombinant DNA Solvents Molecular Medicine Female Immunization Adjuvant hormones hormone substitutes and hormone antagonists |
| Zdroj: | Vaccine. 25(35) |
| ISSN: | 0264-410X |
| Popis: | A recombinant ovalbumin-luteinizing hormone-releasing hormone (ova-LHRH) antigen has been developed for immunocontraception. In this study, a novel immunostimulant for ova-LHRH immunization, CpG oligodeoxynucleotide (ODN) 2006, was compared against Mycobacterium butyricum. Also, the immunogenicity of ova-LHRH after lyophilization and exposure to organic solvents was assessed. Rats received either ova-LHRH solubilized in urea; lyophilized ova-LHRH; lyophilized ova-LHRH exposed to methylene chloride; or lyophilized ova-LHRH exposed to ethyl acetate. Immunogenicity of lyophilized ova-LHRH was reduced compared with solubilized ova-LHRH. Exposure to ethyl acetate further decreased immunogenicity of ova-LHRH. CpG ODN 2006 was a more effective immunostimulant than M. butyricum for LHRH immunization. (C) 2007 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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